Mosahebi G, Ahoon-Manesh A, Sharifi-Tehrani A, Mohammadi M, Okhovvat S M
Agriculture College, University of Tehran.
Commun Agric Appl Biol Sci. 2005;70(3):423-4.
1818 collected samples of potato plant showing virus infection symptoms from 85 fields were tested for PVS infection using DAS-ELISA. Average of infection to this virus varied from 0 to 100%. Least infection was belonging to fields with new introduced varieties. On the other hand native and old introduced cultivars showed heavy infection. In field condition, PVS infected plants didn't show very obvious symptoms, so some infected plants may be missed in field sample collecting. The physical properties of 3 isolates, Avaj, Stanboly and Agria No 15 were determined. TIP 55-60 degrees C, DEP 10(-3) and Liv measured 3-4 days. Ouchterlony agar double diffusion test using SDS was useful for virus detection and precipitation lines didn't show any spur between isolates, although isolates differs slightly in symptomatology. SDS-Page and Western blotting methods used successfully for virus detection and determining and measuring viral protein components.
1818年，从85块田地中采集了表现出病毒感染症状的马铃薯植株样本，使用双抗夹心酶联免疫吸附测定法（DAS - ELISA）检测马铃薯S病毒（PVS）感染情况。该病毒的感染率平均在0%至100%之间。感染率最低的是种植新引进品种的田地。另一方面，本地品种和老引进品种表现出严重感染。在田间条件下，感染PVS的植株没有表现出非常明显的症状，因此在田间样本采集过程中可能会遗漏一些受感染的植株。测定了3个分离株Avaj、Stanboly和Agria No 15的物理特性。热灭活点为55 - 60摄氏度，稀释终点为10（-3），体外存活期为3 - 4天。使用十二烷基硫酸钠（SDS）的琼扩双扩散试验对病毒检测很有用，尽管分离株在症状学上略有不同，但沉淀线在各分离株之间未显示出任何交叉。十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - Page）和蛋白质免疫印迹法成功用于病毒检测以及病毒蛋白成分的测定和测量。
