Mosahebi G, Koohi-Habibi M, Okhovvat S M
College of Agriculture, University of Tehran, Karaj, Iran.
Commun Agric Appl Biol Sci. 2005;70(3):441-3.
57 native potato tuber samples collected from different potato growing region of Iran, planted on single rows in Karaj College experimental station. Plant samples of each single row plus 9.25 Fresh foliage samples collected from fields under new introduced cultivars were tested for potato virus (PVM) infection during growing season. Also 78 weeds and field crops belonging to Solonacae and Leguminosae from or neighboring to potato field were tested. Results indicated that PVM was not found on any plant other than potatoes. PVM was detected on 16 samples of 57 old vars, Virus was not seen in any samples collected from fields under new varieties. Results show that PVM is limiting in this crop. PVM detecting is difficult using assay hosts. Best test plants were French bean var Red kidney, Showing pinpoint necrotic LL, also Datura metel and Nicotiana debneyi are useful for virus detection showing chlorotic local lesion. Also microprecipition and gel diffusion test can be used for virus detection but Elisa was the best method. PVM infected plant showed 11-19.5 percent yield decrease in 3 cultivars tested.
从伊朗不同马铃薯种植区采集的57份本地马铃薯块茎样本，种植在卡拉季学院实验站的单行中。在生长季节，对每行的植株样本以及从新引进品种种植田采集的9.25份新鲜叶片样本进行马铃薯病毒（PVM）感染检测。此外，还对来自马铃薯田或其附近的78种属于茄科和豆科的杂草和大田作物进行了检测。结果表明，除马铃薯外，在任何植物上均未发现PVM。在57个旧品种的16个样本中检测到了PVM，在从新品种种植田采集的任何样本中均未发现该病毒。结果表明，PVM对这种作物具有限制作用。使用检测寄主检测PVM很困难。最佳检测植物是红肾菜豆品种，表现为针尖状坏死局部病斑，曼陀罗和德氏烟草也可用于病毒检测，表现为褪绿局部病斑。此外，微量沉淀和凝胶扩散试验可用于病毒检测，但酶联免疫吸附测定（ELISA）是最佳方法。在测试的3个品种中，感染PVM的植株产量下降了11%-19.5%。
