Angelov Borislav, Angelova Angelina, Papahadjopoulos-Sternberg Brigitte, Lesieur Sylviane, Sadoc Jean-François, Ollivon Michel, Couvreur Patrick
Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl.21, BG-1113 Sofia, Bulgaria.
J Am Chem Soc. 2006 May 3;128(17):5813-7. doi: 10.1021/ja060082c.
Supramolecular three-dimensional self-assembly of nonlamellar lipids with fragments of the protein immunoglobulin results in a bicontinuous cubic phase fragmented into nanoparticles with open water channels (cubosomes). The structure of the diamond-type cubic nanoparticles is characterized experimentally by freeze-fracture electron microscopy, and it is mathematically modeled with nodal surfaces emphasizing the fluid-like undulations of the cubosomic interfaces. Based on scaling-up and scaling-down approaches, we present stable and intermediate-kind nanoparticles resulting from the cubosomic growth. Our results reveal the smallest stable diamond-type cubosomic entity that can serve as a building block of more complex nanostructured fluid drug delivery vehicles of therapeutic proteins. The evidence presented for lipid-bilayer undulations in the surface region of the protein/lipid cubosomes could have important consequences for possible applications of these hierarchically organized porous nanoparticles.
非层状脂质与蛋白质免疫球蛋白片段的超分子三维自组装形成了一种双连续立方相，该相破碎成具有开放水通道的纳米颗粒（立方液晶相脂质体）。通过冷冻断裂电子显微镜对菱形立方纳米颗粒的结构进行了实验表征，并通过强调立方液晶相脂质体界面类似流体波动的节点表面进行了数学建模。基于放大和缩小方法，我们展示了立方液晶相脂质体生长产生的稳定和中间类型的纳米颗粒。我们的结果揭示了最小的稳定菱形立方液晶相脂质体实体，它可以作为更复杂的治疗性蛋白质纳米结构流体药物递送载体的构建块。在蛋白质/脂质立方液晶相脂质体表面区域呈现的脂质双层波动证据可能对这些分层组织的多孔纳米颗粒的可能应用产生重要影响。
