Angelova Angelina, Angelov Borislav, Papahadjopoulos-Sternberg Brigitte, Bourgaux Claudie, Couvreur Patrick
J Phys Chem B. 2005 Mar 3;109(8):3089-93. doi: 10.1021/jp044216p.
Self-assembly lipid/protein cubosomic nanostructures are generated at high hydration level (dispersion of 5% lipid only) and examined by freeze-fracture electron microscopy (FF-EM) and synchrotron X-ray diffraction (XRD). The fracture surface of the three-dimensional (3D) soft-matter membranous assembly reveals starlike nanopatterns of oriented 100-nm-long cubosomic nanoridges with lateral periodicity defined by their 21-nm diameters. The average water channel radius in these liquid crystalline cubosomic nanoarchitectures, determined by high-resolution FF-EM and XRD, is 18.0 Angstrom. The protein-directed fragmentation of a diamond-type lipid cubic phase at high hydration can induce 3D patterns of oriented nanoporous building blocks, which are a unique example of tertiary organization of functionalized fluid lipid/water interfaces.
自组装脂质/蛋白质立方体细胞纳米结构在高水合水平（仅5%脂质分散体）下生成，并通过冷冻断裂电子显微镜（FF-EM）和同步加速器X射线衍射（XRD）进行检测。三维（3D）软物质膜状组装体的断裂表面显示出由直径为21纳米的侧向周期性排列的100纳米长立方体细胞纳米脊组成的星状纳米图案。通过高分辨率FF-EM和XRD确定，这些液晶立方体细胞纳米结构中的平均水通道半径为18.0埃。在高水合状态下，蛋白质导向的菱形脂质立方相断裂可诱导定向纳米多孔构建块的3D图案，这是功能化流体脂质/水界面三级组织的独特实例。
