Angelova Angelina, Angelov Borislav, Papahadjopoulos-Sternberg Brigitte, Ollivon Michel, Bourgaux Claudie
CEP, UMR 8612, University of Paris XI, 5 Rue J.B. Clément, F-92296 Châtenay-Malabry, France.
Langmuir. 2005 Apr 26;21(9):4138-43. doi: 10.1021/la047745t.
Proteocubosomes are nanostructured open-nanochannel hierarchical fluid vehicles characterized by a cubic lattice periodicity of the lipid/protein supramolecular assembly (protein-loaded cubosomes). They are obtained here at very high hydration levels by a three-dimensional (3D) self-assembly process, which exploits a protein-directed 3D patterning and fragmentation to create a new, tertiary-level structural order of fluid lipid/water interfaces. Our freeze-fracture electron microscopy study reveals that the proteocubosome structures are built up by patterned assemblies of nanocubosomes, which comprise 3D nanoporous fracture surfaces throughout. Complex cubosomic architectures, involving arrays of nanodroplets (larger than 20 nm) inside the proteocubosome particles, are established at high resolution. The soft-matter hierarchical nanocompartment formations display internal aqueous pores belonging to the D-type lipid cubic lattice nanochannel system that is proven by synchrotron X-ray diffraction. The reported nanostructured fluid may give rise to novel applications in nanofluidic biomimetic devices, porous protein drug delivery vehicles, nanoscale enzymatic bioreactors, and protein-encapsulating fluid nanomaterials.
蛋白质立方液晶是一种纳米结构的开放纳米通道分级流体载体，其特征在于脂质/蛋白质超分子组装体（载有蛋白质的立方液晶）具有立方晶格周期性。在这里，它们是通过三维（3D）自组装过程在非常高的水合水平下获得的，该过程利用蛋白质导向的3D图案化和碎片化来创建流体脂质/水界面的新的三级结构顺序。我们的冷冻断裂电子显微镜研究表明，蛋白质立方液晶结构是由纳米立方液晶的图案化组装构建而成的，纳米立方液晶在整个过程中都包含3D纳米多孔断裂表面。在高分辨率下建立了复杂的立方液晶结构，包括蛋白质立方液晶颗粒内的纳米液滴（大于20nm）阵列。软物质分级纳米隔室形成显示出属于D型脂质立方晶格纳米通道系统的内部水孔，这已通过同步加速器X射线衍射得到证实。报道的纳米结构流体可能会在纳米流体仿生装置、多孔蛋白质药物递送载体、纳米级酶生物反应器和蛋白质封装流体纳米材料等方面产生新的应用。
