Darmani H, Nusayr T, Al-Hiyasat A S
Department of Applied Biology, Faculty of Science, Jordan University of Science and Technology, Irbid, Jordan.
Int J Dent Hyg. 2006 May;4(2):62-6. doi: 10.1111/j.1601-5037.2006.00149.x.
This study examined the effects of extracts of two chewing sticks on proliferation of fibroblasts and viability of cariogenic bacteria.
Aqueous extracts of miswak (Salvadora persica; Arak tree) and derum (Juglans regia; walnut tree) were prepared and their effects investigated on growth of Balb/C 3T3 mouse fibroblasts by measuring the mitochondrial succinic dehydrogenase activity. Furthermore, the effects on the viability of various cariogenic bacteria (Streptococcus mutans, Streptococcus salivarius, Lactobacillus casei and Actinomyces viscosus) was also determined.
The data revealed that Balb/C 3T3 fibroblasts exposed to aqueous extracts of miswak or derum showed an increase in cell proliferation by 156% and 255%, respectively, in comparison with controls (p < 0.0001). Furthermore, extracts from both miswak and derum had adverse effects on the growth of the cariogenic microorganisms, with derum having significantly greater antimicrobial effects than miswak and at much lower concentrations against all the bacteria tested. The most sensitive organisms were A. viscosus, followed by S. mutans, S. salivarius, with L. casei being the most resistant.
The results show that aqueous extracts of miswak and derum enhance the growth of fibroblasts and inhibit the growth of cariogenic bacteria, with the derum extract showing greater activity than miswak.
本研究考察了两种咀嚼棒提取物对成纤维细胞增殖和致龋菌活力的影响。
制备了阿拉伯树胶（ Salvadora persica；阿拉伯树）和胡桃树（ Juglans regia；核桃树）的水提取物，并通过测量线粒体琥珀酸脱氢酶活性来研究它们对Balb/C 3T3小鼠成纤维细胞生长的影响。此外，还测定了其对各种致龋菌（变形链球菌、唾液链球菌、干酪乳杆菌和粘性放线菌）活力的影响。
数据显示，与对照组相比，暴露于阿拉伯树胶水提取物或胡桃树水提取物的Balb/C 3T3成纤维细胞的细胞增殖分别增加了156%和255%（p < 0.0001）。此外，阿拉伯树胶和胡桃树的提取物对致龋微生物的生长均有不利影响，胡桃树提取物的抗菌作用明显大于阿拉伯树胶，且在低得多的浓度下对所有测试细菌均有抗菌作用。最敏感的微生物是粘性放线菌，其次是变形链球菌、唾液链球菌，干酪乳杆菌最具抗性。
结果表明，阿拉伯树胶和胡桃树的水提取物可促进成纤维细胞生长并抑制致龋菌生长，胡桃树提取物的活性高于阿拉伯树胶。
