Movahhed Taraneh, Mehrabkhani Maryam, Arefnezhad Mohsen, Hamedi Shokouh Sadat, Zare Mahmoudabadi Reza, Ghanbari Fariba, Rostami Mahjubeh
Dental School, Mashhad University of Medical Sciences, Iran.
Kashmar School of Nursing, Mashhad University of Medical Sciences, Iran.
Rep Biochem Mol Biol. 2022 Oct;11(3):405-410. doi: 10.52547/rbmb.11.3.405.
Chemical agents, such as Chlorhexidine are used as one of dental plaque control strategy. Researchers are looking for a natural and economic substitute with same antibacterial efficacy and less complications. The aim of this study was to evaluate the antimicrobial efficacy of the Khorasan Razavi walnut green husk (WGH) extract with and without adding ZnO nanoparticles (nZnO) on ().
In this study, antimicrobial effect of the Hydro-ethanolic extract of WGH, was evaluated against . Broth Dilution and Agar diffusion methods were used with 90 tubes containing different dilutions of WGH extract (100 to 0.006 mg/ml). ZnO nanoparticles (nZnO) were added to 45 tubes. was exposed to 15 different serial concentrations of study extracts, from 100 mg/ml to 0.006 mg/ml. Minimum inhibitory concentration (MIC) of the study extracts were determined and zone of inhibition diameter was compared to positive controls (chlorhexidine 0.2%, nZnO), and negative control (sterile distilled water). The differences between the mean diameters, were analyzed by independent sample T- teS.
Minimum inhibitory concentration (MIC) of study extract was found to be 50mg/mL, with adding nZnO, MIC was reduced to 3.12mg/mL. Mean diameter of inhibition zone at 3.12 mg/ml with and without adding ZnO nanoparticles were 17.67±0.57 mm and 8±0.001 mm, respectively, (p-value< 0.001).
Adding nZnO could be enhanced antimicrobial efficacy of the WGH extract against , while it was still less effective than chlorhexidine.
化学药剂，如氯己定，被用作控制牙菌斑的策略之一。研究人员正在寻找一种具有相同抗菌效果且并发症更少的天然且经济的替代品。本研究的目的是评估添加和不添加氧化锌纳米颗粒（nZnO）的霍拉桑拉扎维核桃青皮（WGH）提取物对（）的抗菌效果。
在本研究中，评估了WGH水乙醇提取物对（）的抗菌效果。采用肉汤稀释法和琼脂扩散法，使用90支含有不同稀释度WGH提取物（100至0.006mg/ml）的试管。向45支试管中添加了氧化锌纳米颗粒（nZnO）。（）暴露于15种不同系列浓度的研究提取物中，浓度范围为100mg/ml至0.006mg/ml。测定研究提取物的最低抑菌浓度（MIC），并将抑菌圈直径与阳性对照（0.2%氯己定、nZnO）和阴性对照（无菌蒸馏水）进行比较。通过独立样本T检验分析平均直径之间的差异。
研究提取物的最低抑菌浓度（MIC）为50mg/mL，添加nZnO后，MIC降至3.12mg/mL。在3.12mg/ml时，添加和不添加氧化锌纳米颗粒的抑菌圈平均直径分别为17.67±0.57mm和8±0.001mm，（p值<0.001）。
添加nZnO可增强WGH提取物对（）的抗菌效果，但其效果仍低于氯己定。
