墨西哥牛分枝杆菌临床分离株中PE/PE_PGRS序列的PE结构域多态性

Polymorphism of the PE domain of PE/PE_PGRS sequences in clinical isolates of Mycobacterium bovis in Mexico.

作者信息

Santillan-Flores Marco Antonio, Flores Javier, Arriaga-Diaz Camila, Romero-Torres Cecilia, Suárez-Güemes Francisco, Espitia Clara

机构信息

Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, CENID-Microbiología, Mexico City, Mexico.

出版信息

Vet Microbiol. 2006 Jul 20;115(4):364-9. doi: 10.1016/j.vetmic.2006.02.021. Epub 2006 Apr 25.

DOI:10.1016/j.vetmic.2006.02.021

PMID:16638629

Abstract

Polymorphism of the PE domain of PE/PE_PGRS sequences was studied in Mycobacterium bovis isolates from different Mexican states. Samples were analyzed by spolygotyping and RFLP using IS6110 and a 235-bp fragment of the PE domain of PE/PE_PGRS as probes. With the PE probe, three different genotypes were observed, one being predominant in all states. These results confirm the high conservation of the PE domain and suggests a potential role for PE sequence as a stable genetic marker for bovine tuberculosis.

摘要

对来自墨西哥不同州的牛分枝杆菌分离株中PE/PE_PGRS序列的PE结构域多态性进行了研究。使用IS6110以及PE/PE_PGRS的PE结构域的一个235 bp片段作为探针，通过spolygotyping和RFLP对样本进行分析。使用PE探针时，观察到三种不同的基因型，其中一种在所有州都占主导地位。这些结果证实了PE结构域的高度保守性，并表明PE序列作为牛结核病稳定遗传标记具有潜在作用。