McNally James B, Kirkpatrick Nathaniel D, Hariri Lida P, Tumlinson Alexandre R, Besselsen David G, Gerner Eugene W, Utzinger Urs, Barton Jennifer K
University of Arizona, Tuscon 85721-0104, USA.
Appl Opt. 2006 May 1;45(13):3049-62. doi: 10.1364/ao.45.003049.
Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The Apc(Min/+) genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680 nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680 nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.
光学相干断层扫描（OCT）、激光诱导荧光（LIF）和激光扫描共聚焦显微镜（LSCM）被用于对健康和腺瘤性小鼠结肠进行多模态研究。将每种模态的结果与作为金标准的组织学结果进行比较。将结肠癌的Apc(Min/+)基因小鼠模型与野生型小鼠进行比较。此外，采用特殊饮食来研究先前在结肠中观察到的680 nm自发荧光信号的来源。研究发现每种模态之间以及与组织学结果都有密切的一致性。所有四种模态都能够准确识别病变组织。OCT和LSCM图像提供了关于组织的互补结构信息，而LIF和LSCM测量的自发荧光信号提供了生化信息。OCT和LIF是在体内进行且无损检测，而LSCM和组织学需要提取组织。680 nm信号的强度与小鼠饮食中的叶绿素含量相关，表明自发荧光化合物是一种饮食代谢产物。
