Fang Weihuan, Budde Birgitte Bjørn, Siegumfeldt Henrik
College of Animal Sciences, Zhejiang University, Zhejiang, China.
FEMS Microbiol Lett. 2006 May;258(2):208-13. doi: 10.1111/j.1574-6968.2006.00219.x.
A mixed culture of single cells of Listeria monocytogenes and the bacteriocin producing Leuconostoc carnosum 4010 showed growth inhibition of L. monocytogenes, although the intracellular pH (pHi) of L. monocytogenes followed by fluorescence ratio imaging microscopy was not affected. Furthermore, L. monocytogenes was exposed to the bacteriocins leucocins 4010 and nisin either in a liquid filled chamber or on the surface of an agar containing bacteriocins. Both bacteriocins caused dissipation of the pH gradient in L. monocytogenes and the effect was clearly dependent on the matrix, as the decrease in pHi occurred much more rapidly in liquid than in agar.
单核细胞增生李斯特菌的单细胞与产细菌素的肉糖肠球菌4010的混合培养物显示出对单核细胞增生李斯特菌的生长抑制作用，尽管通过荧光比率成像显微镜观察到的单核细胞增生李斯特菌的细胞内pH值（pHi）并未受到影响。此外，单核细胞增生李斯特菌在充满液体的小室中或含有细菌素的琼脂表面接触细菌素亮菌素4010和乳链菌肽。两种细菌素均导致单核细胞增生李斯特菌的pH梯度消散，并且该效应明显取决于基质，因为pHi的降低在液体中比在琼脂中发生得更快。
