Lanzi Gaetana, de Miranda Joachim R, Boniotti Maria Beatrice, Cameron Craig E, Lavazza Antonio, Capucci Lorenzo, Camazine Scott M, Rossi Cesare
Reparto di Biologia Moleculare, Istituto Zooprofilattico Sperimentale Lombardia e Emilia-Romagna, Brescia, Italy 25124-I.
J Virol. 2006 May;80(10):4998-5009. doi: 10.1128/JVI.80.10.4998-5009.2006.
Deformed wing virus (DWV) of honeybees (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. The virus was purified from diseased insects, and its genome was cloned and sequenced. The genomic RNA of DWV is 10,140 nucleotides in length and contains a single large open reading frame encoding a 328-kDa polyprotein. The coding sequence is flanked by a 1,144-nucleotide 5' nontranslated leader sequence and a 317-nucleotide 3' nontranslated region, followed by a poly(A) tail. The three major structural proteins, VP1 (44 kDa), VP2 (32 kDa), and VP3 (28 kDa), were identified, and their genes were mapped to the N-terminal section of the polyprotein. The C-terminal part of the polyprotein contains sequence motifs typical of well-characterized picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease, and an RNA-dependent RNA polymerase. The genome organization, capsid morphology, and sequence comparison data indicate that DWV is a member of the recently established genus Iflavirus.
蜜蜂的变形翅病毒（DWV）与特征性的翅畸形、腹部肿胀、麻痹以及新羽化成年蜜蜂的快速死亡密切相关。该病毒从患病昆虫中纯化出来，其基因组被克隆并测序。DWV的基因组RNA长度为10,140个核苷酸，包含一个单一的大开放阅读框，编码一个328 kDa的多聚蛋白。编码序列两侧是一个1,144个核苷酸的5'非翻译前导序列和一个317个核苷酸的3'非翻译区，随后是一个聚腺苷酸尾巴。鉴定出了三种主要结构蛋白，VP1（44 kDa）、VP2（32 kDa）和VP3（28 kDa），并将它们的基因定位到多聚蛋白的N端部分。多聚蛋白的C端部分包含特征明确的小RNA病毒非结构蛋白的典型序列基序：一种RNA解旋酶、一种胰凝乳蛋白酶样3C蛋白酶和一种RNA依赖性RNA聚合酶。基因组结构、衣壳形态和序列比较数据表明，DWV是最近建立的伊弗病毒属的成员。
