Hesketh-Best Poppy J, Mckeown Dean A, Christmon Krisztina, Cook Steven, Fauvel Anne Marie, Steinhauer Nathalie A, Schroeder Declan C
Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN, USA.
Bee Informed Partnership, College Park, MD, USA.
Commun Biol. 2024 Dec 5;7(1):1623. doi: 10.1038/s42003-024-07333-9.
Honey bees are essential pollinators for global agriculture. The viromes of US commercial apiaries and their ectoparasitic mites are poorly characterized at a strain level and there is a need to integrate genomics into pathogen surveillance. We sequenced RNA viromes from 383 adult bees and 173 mites pooled samples from 11 major US beekeeping hubs in 2021, assembling 45 complete and 1702 partial genomes. Protein sequence similarity networks and recombinant genome identification revealed a new viral landscape. Sinaivirus (n = 312), Iflavirus sacbroodi (n = 280), and Iflavirus aladeformis (DWV, n = 135) genomes were common. Recombinant DWV genomes with high nucleotide identity were widespread, and DWV type A master variants were rare, with an indication that RT-PCR surveillance may over-detect type A due to the prevalence of recombinant DWV genomes. Future work should use genomic strategies to avoid misidentification of common honey bee virus genomes and their impact on colony health.
蜜蜂是全球农业至关重要的传粉者。美国商业养蜂场及其外寄生螨的病毒组在菌株水平上的特征描述不足，因此有必要将基因组学纳入病原体监测。我们对2021年来自美国11个主要养蜂中心的383只成年蜜蜂和173只螨的混合样本进行了RNA病毒组测序，组装了45个完整基因组和1702个部分基因组。蛋白质序列相似性网络和重组基因组鉴定揭示了一个新的病毒格局。西奈病毒（n = 312）、囊状幼虫病毒（n = 280）和变形翅病毒（DWV，n = 135）的基因组很常见。具有高核苷酸同一性的重组DWV基因组广泛存在，而A型DWV主要变体很少见，这表明由于重组DWV基因组的普遍存在，RT-PCR监测可能会过度检测到A型。未来的工作应采用基因组策略，以避免对常见蜜蜂病毒基因组的错误识别及其对蜂群健康的影响。
