Sanchez Juan M, Sacks Richard D
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, and Department of Chemistry, University of Girona, 17071-Girona, Spain.
Anal Chem. 2006 May 1;78(9):3046-54. doi: 10.1021/ac060053k.
A method for the determination of volatile organic compounds (VOCs) at sub-trace levels in breath samples based on a multibed sorption trap for the collection and concentration of VOCs, a comprehensive multidimensional gas chromatograph (GCxGC) for the separation of complex mixtures, and a time-of-flight mass spectrometer detector is designed and developed. The good performance of the trap tube device developed for the concentration together with the high sensitivity and separation power of the GCxGC results in a powerful system. In the analysis of samples, more than 100 different compounds are detected of which between 65 and 85 are clearly identified. A total of approximately 250 different compounds are observed in all the samples evaluated of which 142 are identified. A preliminary study to evaluate breath biomarkers for active smoking is performed. The levels of previously described biomarkers are found to be strongly time-dependent with amounts found approximately 1 h after smoking returning to the levels found in nonsmoking volunteers. However, 2,5-dimethylfuran, 2-methylfuran, and furan are found to be effective biomarkers given that they were only found in samples taken from smokers and could still be detected more than 2 h after smoking.
设计并开发了一种基于多床吸附阱用于采集和浓缩挥发性有机化合物（VOCs）、全二维气相色谱仪（GCxGC）用于分离复杂混合物以及飞行时间质谱仪检测器来测定呼出气体样本中亚痕量水平VOCs的方法。为浓缩而开发的捕集管装置的良好性能，以及GCxGC的高灵敏度和分离能力，造就了一个强大的系统。在样本分析中，检测到100多种不同的化合物，其中65至85种被明确鉴定。在所有评估的样本中总共观察到约250种不同的化合物，其中142种被鉴定。开展了一项评估主动吸烟呼出生物标志物的初步研究。发现先前描述的生物标志物水平强烈依赖时间，吸烟后约1小时测得的量恢复到非吸烟志愿者中测得的水平。然而，2,5 - 二甲基呋喃、2 - 甲基呋喃和呋喃被发现是有效的生物标志物，因为它们仅在吸烟者的样本中被发现，并且在吸烟后2小时以上仍可检测到。
