Department of Anesthesiology and Intensive Care Medicine, University Rostock, Schillingallee 35, 18057 Rostock, Germany.
Anal Chem. 2010 Mar 15;82(6):2541-51. doi: 10.1021/ac100061k.
This study was intended to evaluate low-volume (20 mL) multibed needle trap (NTD) sampling combined with heart-cut gas chromatography/mass spectrometry (GC/MS) and comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC x GC/TOF-MS) for trace gas analysis under clinical conditions. NTDs, high-throughput automatic desorption and separation systems, were tested in vitro and within a study in 11 patients undergoing cardiac surgery with respect to reproducibility, reliability, and clinical applicability. NTD-heart-cut GC/MS analysis of standard mixtures containing different volatile organic compounds (VOCs) yielded relative standard deviations (RSDs) from 4.0% to 18.5%. Substance adsorption was stable for 1 day if NTDs were closed on both ends and was stable for approximately 7.8 h when NTD tip ends had to be left open during autosampler storage. Even in the presence of high concentrations of contaminants linearity of heart-cut GC/MS was conserved. In patients' breath potential biomarkers could be determined even in the presence of very high concentrations of sevoflurane. Profiles of blood-borne biomarkers, intravenous drugs, and clinical contaminants were characterized. Comprehensive GC x GC/TOF-MS may be used as a screening tool for new biomarkers, if patterns are generated from deconvoluted normalized areas. Needle trap sampling in combination with hyphenated chromatographic techniques can thus be used to provide well-tailored solutions for complex problems occurring in clinical breath analysis.
本研究旨在评估在临床条件下，使用小体积（20 毫升）多床针阱（NTD）采样结合心切割气相色谱/质谱（GC/MS）和全面二维气相色谱/飞行时间质谱（GC x GC/TOF-MS）进行痕量气体分析。NTD、高通量自动解吸和分离系统在体外和 11 例接受心脏手术的患者中进行了测试，以评估其重现性、可靠性和临床适用性。NTD-心切割 GC/MS 分析含有不同挥发性有机化合物（VOC）的标准混合物，得到的相对标准偏差（RSD）为 4.0%至 18.5%。如果 NTD 的两端都封闭，则 NTD 的物质吸附稳定 1 天，如果在自动进样器存储过程中必须打开 NTD 的尖端，则稳定约 7.8 小时。即使存在高浓度的污染物，心切割 GC/MS 的线性也得以保留。即使在七氟醚浓度非常高的情况下，也可以在患者的呼吸中确定潜在的生物标志物。血液传播生物标志物、静脉内药物和临床污染物的特征得到了描述。如果从解卷积归一化面积生成模式，则综合 GC x GC/TOF-MS 可用作新生物标志物的筛选工具。因此，针阱采样与色谱技术联用可用于为临床呼吸分析中出现的复杂问题提供量身定制的解决方案。
