Imai Koichi, Nakamura Masaaki
Department of Biomaterials, Osaka Dental University, Hirakata-city, Osaka, Japan.
Congenit Anom (Kyoto). 2006 Mar;46(1):34-8. doi: 10.1111/j.1741-4520.2006.00099.x.
We examined embryotoxicity using the embryonic stem cell test (EST) protocol. Tests were conducted using standard reagents for the atomic absorption measurement of 11 metal ions, silver, cobalt, chromium, copper, mercury, nickel, palladium, antimony, tin, vanadium, and zinc from among metals comprising dental alloys. In addition, for four metals like silver, cobalt, chromium, and nickel, the tests were also conducted using a test solution extracted from powder in the cell culture medium. The embryotoxic potential was obtained from a biostatistics-based prediction model, which was calculated from three endpoints, the ID50, IC50ES and IC(50)3T3. Data with the standard reagents showed that chromium and mercury ions corresponded to class 3, that is, having a strong embryotoxicity, while antimony, tin, and vanadium ions exhibited a weak embryotoxicity. The other metal ions demonstrated no embryotoxicity. On the other hand, when extracts of metal powder in cell culture solutions were used, silver exhibited a weak embryotoxicity while all other metals exhibited no embryotoxicity. In the future, it will be important to clarify the embryotoxicity of the many dental materials that are in use today. In addition, it is necessary to develop substances to ensure they have no toxicity before use in dental applications.
我们使用胚胎干细胞试验（EST）方案检测胚胎毒性。使用标准试剂对牙科合金所含的11种金属离子（银、钴、铬、铜、汞、镍、钯、锑、锡、钒和锌）进行原子吸收测量测试。此外，对于银、钴、铬和镍这四种金属，还使用从细胞培养基中的粉末提取的测试溶液进行了测试。胚胎毒性潜力是通过基于生物统计学的预测模型获得的，该模型由三个终点指标计算得出，即半数抑制浓度（ID50）、胚胎干细胞半数抑制浓度（IC50ES）和3T3细胞半数抑制浓度（IC50）3T3。使用标准试剂的数据表明，铬离子和汞离子属于3类，即具有强胚胎毒性，而锑、锡和钒离子表现出弱胚胎毒性。其他金属离子未显示出胚胎毒性。另一方面，当使用细胞培养液中金属粉末的提取物时，银表现出弱胚胎毒性，而所有其他金属均未表现出胚胎毒性。未来，明确当今使用的众多牙科材料的胚胎毒性将很重要。此外，有必要开发在牙科应用中使用前确保无毒的物质。
