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牙科生物材料胚胎毒性技术的发展

Development of technique for embryotoxicity of dental biomaterials.

作者信息

Imai Koichi

机构信息

Department of Biomaterials, Osaka Dental University, 8-1, Kuzuha Hanazono-cho, Hirakata, Osaka 573-1121, Japan.

出版信息

Jpn Dent Sci Rev. 2016 Aug;52(3):54-62. doi: 10.1016/j.jdsr.2016.02.002. Epub 2016 Jun 11.

DOI:10.1016/j.jdsr.2016.02.002
PMID:28408957
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5390336/
Abstract

The Embryonic Stem Cell Test (EST) developed in Germany in 1997 is known as a screening test method capable of predicting the presence of unknown chemicals influencing normal human development. Firstly, we investigated the embryotoxicity of 24 types of monomer including dental monomers and dental alloy-component metal elements using this test. Monomers including Bis-GMA contained in base resin of composite resin exhibited weak embryotoxicity, and the toxicity level varied among dental alloy-component metal elements. It was clarified that metal ions eluted from currently sold dental alloys show no embryotoxicity. Then, we investigated a method that also considers human metabolic activity, which is not possible with the EST, in the results of embryotoxicity. In addition, an evaluation method using a hybrid culture system for hepatocytes and mouse ES cells and a method using oviduct or uterus cells for feeder cells were also investigated.

摘要

1997年在德国开发的胚胎干细胞试验（EST）是一种能够预测影响人类正常发育的未知化学物质存在的筛选试验方法。首先，我们使用该试验研究了24种单体的胚胎毒性，这些单体包括牙科单体和牙科合金成分金属元素。复合树脂基础树脂中含有的双酚A-甲基丙烯酸缩水甘油酯（Bis-GMA）等单体表现出较弱的胚胎毒性，并且牙科合金成分金属元素的毒性水平各不相同。已明确，目前销售的牙科合金洗脱的金属离子没有胚胎毒性。然后，我们研究了一种在胚胎毒性结果中也考虑人类代谢活性的方法，而胚胎干细胞试验无法做到这一点。此外，还研究了一种使用肝细胞和小鼠胚胎干细胞的混合培养系统的评估方法以及一种使用输卵管或子宫细胞作为饲养细胞的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/d1357568dfcd/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/3ccb9973f935/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/675225a18490/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/9c578c9f4c95/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/1cdf0658ba1d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/81dc143467d9/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/aedea7aa026b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/4e12bd2634bc/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/49b1e4728909/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/d1357568dfcd/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/3ccb9973f935/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/675225a18490/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/9c578c9f4c95/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/1cdf0658ba1d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/81dc143467d9/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/aedea7aa026b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/4e12bd2634bc/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/49b1e4728909/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/5390336/d1357568dfcd/gr9.jpg

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本文引用的文献

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The validated embryonic stem cell test to predict embryotoxicity in vitro.经验证的胚胎干细胞测试可预测体外胚胎毒性。
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Altern Lab Anim. 2006 Oct;34(5):527-38. doi: 10.1177/026119290603400504.
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In vitro embryotoxicity testing of metals for dental use by differentiation of embryonic stem cell test.
通过胚胎干细胞试验分化进行牙科用金属的体外胚胎毒性测试。
Congenit Anom (Kyoto). 2006 Mar;46(1):34-8. doi: 10.1111/j.1741-4520.2006.00099.x.
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