Cheng Pei-Wen, Ng Lean-Teik, Lin Chun-Ching
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, No. 100, Shin-Chuan 1st Road, Kaohsiung 807, Taiwan.
Int Immunopharmacol. 2006 Jun;6(6):1003-12. doi: 10.1016/j.intimp.2006.01.011. Epub 2006 Feb 21.
Coxsackie B virus type 1 (CVB1) infection is known to cause high morbidity and mortality in children, however, there is no effective drug for treating this disease. The present study aimed to examine the antiviral activity of xiao chai hu tang (XCHT), a popular herbal drug for treating viral and bacterial infections, against CVB1 infection and its mechanisms of action. Our data showed that XCHT neutralized the CVB1-induced cytopathic effect in human neonatal foreskin fibroblast cell line (CCFS-1/KMC), with IC50 (virus-induced cytopathic effect by 50%) and EC50 (concentration of 50% effectiveness) values around 12.39 and 50.93 microg/ml, respectively. Its CC50 (concentration of 50% cellular cytotoxicity) and SI (selective index) values were 945.75 microg/ml and 18.92, respectively. These results suggest that XCHT possessed anti-CVB1 activity, and showed no effect on CCFS-1 cell viability and growth at concentration 250 microg/ml. The time-of-addition studies showed that XCHT (50, 100 and 200 microg/ml) added at various time of preinfection (-1 to -3 h), coinfection (0 h) and postinfection (1 approximately 3 h) could inhibit CVB1 infection. Interestingly, XCHT also showed an inhibition on viral replication through the induction of IFN-alpha/beta expression. In conclusion, XCHT possessed antiviral activity against CVB1 infection. It interfered the early stage of viral replication (prophylactic effect) and viral replication after infection (therapeutic effect) through the induction of Type I interferon expression.
已知1型柯萨奇B病毒（CVB1）感染会导致儿童高发病率和死亡率，然而，目前尚无治疗该疾病的有效药物。本研究旨在检测小柴胡汤（XCHT）（一种用于治疗病毒和细菌感染的常用草药）对CVB1感染的抗病毒活性及其作用机制。我们的数据显示，XCHT可中和CVB1在人新生儿包皮成纤维细胞系（CCFS-1/KMC）中诱导的细胞病变效应，其IC50（50%病毒诱导细胞病变效应）和EC50（50%有效浓度）值分别约为12.39和50.93微克/毫升。其CC50（50%细胞毒性浓度）和SI（选择性指数）值分别为945.75微克/毫升和18.92。这些结果表明，XCHT具有抗CVB1活性，且在250微克/毫升浓度下对CCFS-1细胞活力和生长无影响。加药时间研究表明，在感染前（-1至-3小时）、共感染（0小时）和感染后（1至3小时）的不同时间添加XCHT（50、100和200微克/毫升）均可抑制CVB1感染。有趣的是，XCHT还可通过诱导IFN-α/β表达抑制病毒复制。总之，XCHT具有抗CVB1感染的抗病毒活性。它通过诱导I型干扰素表达干扰病毒复制的早期阶段（预防作用）和感染后的病毒复制（治疗作用）。
