Chang Jung San, Wang Kuo Chih, Chiang Lien Chai
Faculty of Renal Care, College of Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan.
J Ethnopharmacol. 2008 Sep 2;119(1):104-8. doi: 10.1016/j.jep.2008.06.004. Epub 2008 Jun 13.
Sheng-Ma-Ge-Gen-Tang (SMGGT), a popular prescription of Chinese traditional medicine, has been used to manage measles infection of children for thousands of years. There are evidences to presume a wider spectrum of antiviral activity of SMGGT. However, SMGGT has not been proven to have activity against EV71 infection.
We tested the hypothesis that SMGGT could inhibit cytotoxic effect of EV71.
Human foreskin fibroblast cell line was used for viral culture. Cytotoxicity was examined by XTT assay.
SMGGT could inhibit cytopathy induced by EV71 when given before (p<0.0001), in association with (p<0.0001), or after viral infection (p<0.0001). SMGGT was effective (IC(50): 0.21 microg/ml) and safe (SI: more than 24,000). SMGGT could inhibit viral attachment (p<0.0001) and penetration (p<0.0001). EV71 infection could induce cellular interferon production (p<0.0001). However, SMGGT affected neither the virus-induced (p=0.9913), nor the constitutional interferon production (p>0.05). Therefore, SMGGT had direct anti-viral activity not mediated by interferon.
SMGGT was effective on management of the disease induced by EV71 infection.
升麻葛根汤（SMGGT）是一种常用的中药方剂，数千年来一直用于治疗儿童麻疹感染。有证据推测SMGGT具有更广泛的抗病毒活性。然而，尚未证实SMGGT对肠道病毒71型（EV71）感染有活性。
我们检验了SMGGT可抑制EV71细胞毒性作用的假设。
用人包皮成纤维细胞系进行病毒培养。通过XTT法检测细胞毒性。
在病毒感染前（p<0.0001）、感染时（p<0.0001）或感染后（p<0.0001）给予SMGGT均可抑制EV71诱导的细胞病变。SMGGT有效（半数抑制浓度（IC50）：0.21微克/毫升）且安全（安全指数：超过24,000）。SMGGT可抑制病毒附着（p<0.0001）和穿透（p<0.0001）。EV71感染可诱导细胞产生干扰素（p<0.0001）。然而，SMGGT既不影响病毒诱导的干扰素产生（p=0.9913），也不影响内源性干扰素产生（p>0.05）。因此，SMGGT具有不依赖干扰素介导的直接抗病毒活性。
SMGGT对EV71感染所致疾病的治疗有效。
