Manna S, Bhattacharyya D, Mandal T K, Dey S
Institute of Animal Health and Veterinary Biologicals (R&T), 37-Belgachia Rd, Kolkata-700 037, India.
J Vet Sci. 2006 Jun;7(2):133-6. doi: 10.4142/jvs.2006.7.2.133.
This study examined the effect of deltamethrin on some of the neuropharmacological paradigms in a rat brain such as the motor co-ordination test using a rotarod, the pentobarbitone-induced sleeping time and pentylenetetrazole (PTZ)-induced convulsion as well as the gamma aminobutyric acid (GABA) level. Albino Wistar rats were used as the experimental animals. Different neuropharmacological paradigms such as the motor co-ordination by the rotarod, pentobarbitone-induced sleeping time and the PTZinduced convulsion were examined after administering deltamethrin orally at two doses, 150 mg/kg (LD50) and 15 mg/kg (1/10 LD50). The GABA level in the rat brain was estimated by HPLC after a single oral dose of 150 mg/kg deltamethrin. Deltamethrin significantly reduced the motor coordination, decreased the onset time and increased the sleeping time duration induced by pentobarbitone. In addition, it also decreased the onset time and increased the duration of convulsions induced by PTZ at 150 mg/kg (LD50) and 15 mg/kg (1/10 LD50), respectively. Further deltamethrin administration decreased the GABA levels in the cerebellum as well as in the whole brain (except the cerebellum) significantly at the LD50 dose level. There was some correlation between the effect of deltamethrin on the central GABA levels and its neuropharmacological effects.
本研究考察了溴氰菊酯对大鼠脑内一些神经药理学指标的影响，如使用转棒进行的运动协调测试、戊巴比妥诱导的睡眠时间和戊四氮（PTZ）诱导的惊厥，以及γ-氨基丁酸（GABA）水平。白化Wistar大鼠作为实验动物。在口服给予溴氰菊酯150 mg/kg（半数致死量）和15 mg/kg（1/10半数致死量）两个剂量后，检测了不同的神经药理学指标，如转棒测试的运动协调性、戊巴比妥诱导的睡眠时间和PTZ诱导的惊厥。在单次口服150 mg/kg溴氰菊酯后，通过高效液相色谱法（HPLC）测定大鼠脑内的GABA水平。溴氰菊酯显著降低了运动协调性，缩短了戊巴比妥诱导的入睡时间并延长了睡眠时间。此外，在150 mg/kg（半数致死量）和15 mg/kg（1/10半数致死量）时，它还分别缩短了PTZ诱导惊厥的发作时间并延长了惊厥持续时间。进一步研究发现，在半数致死量剂量水平下，溴氰菊酯给药显著降低了小脑以及全脑（小脑除外）的GABA水平。溴氰菊酯对中枢GABA水平的影响与其神经药理学效应之间存在一定相关性。
