Ohno Yusuke, Kihara Akio, Sano Takamitsu, Igarashi Yasuyuki
Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12-jo, Nishi 6-choume, Kita-ku, Sapporo 060-0812, Japan.
Biochim Biophys Acta. 2006 Apr;1761(4):474-83. doi: 10.1016/j.bbalip.2006.03.010. Epub 2006 Apr 7.
Increasing evidence indicates that DHHC cysteine-rich domain-containing proteins (DHHC proteins) are protein acyltransferases. Although multiple DHHC proteins are found in eukaryotes, characterization has been examined for only a few. Here, we have cloned all the yeast and human DHHC genes and investigated their intracellular localization and tissue-specific expression. Most DHHC proteins are localized in the ER and/or Golgi, with a few localized in the plasma membrane and one in the yeast vacuole. Human DHHC mRNAs also differ in their tissue-specific expression. These results may provide clues to aid in discovering the specific function(s) of each DHHC protein.
越来越多的证据表明，含DHHC富半胱氨酸结构域的蛋白质（DHHC蛋白）是蛋白质酰基转移酶。尽管在真核生物中发现了多种DHHC蛋白，但仅对少数几种进行了特性研究。在此，我们克隆了所有酵母和人类的DHHC基因，并研究了它们的细胞内定位和组织特异性表达。大多数DHHC蛋白定位于内质网和/或高尔基体，少数定位于质膜，一种定位于酵母液泡。人类DHHC mRNA在组织特异性表达上也存在差异。这些结果可能为发现每种DHHC蛋白的特定功能提供线索。
