Yang Jen Ming, Yang Shu Jyuan, Lin Hao Tzu, Chen Jan Kan
Department of Chemical and Materials Engineering, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan 33302, Republic of China.
J Biomed Mater Res B Appl Biomater. 2007 Jan;80(1):43-51. doi: 10.1002/jbm.b.30566.
Hydroxyl-terminated polybutadiene (HTPB)-based polyurethane with dimethyol propionic acid (DPA) as chain extender was synthesized by solution polymerization. The HTPB-based polyurethane was modified by UV radiation with N-isopropyl acrylamide monomer to get poly(N-isopropyl acrylamide)-modified polyurethane (PUDPANIPAAm). The cohesive energy (E(coh)), molar volume (V), solubility parameter (delta), molecular weight (W(M)), volume per gram (V(g)), and the density (1/V(g)) of PUDPANIPAAm were calculated by group contribution methods. To evaluate the application of PUDPANIPAAm for wound dressing and transplantation of cell sheet, the measurement of water content, water vapor transmission rate, and gas permeation on the PUDPANIPAAm membrane was evaluated. The biocompatibility of these membranes, cell adhesion, and proliferation assay were conducted in the cell culture. The effect of thermosensitivity of poly(N-isopropyl acrylamide) on cell detachment was also evaluated in the primary study. The results showed that these PUDPANIPAAm membranes are thermosensitive. The modification of PU with poly(N-isopropyl acrylamide) reduced the water vapor transmission rate and permeability of gas through PUDPANIPAAm membrane. PUDPANIPAAm membranes could support cell adhesion and growth. Owing to the thermosensitive nature of poly(N-isopropyl acrylamide), the relative cell numbers detached from PUDPANIPAAm membranes were larger than those detached from the polystyrene dish.
以端羟基聚丁二烯（HTPB）为基础、二羟甲基丙酸（DPA）为扩链剂，通过溶液聚合反应合成了聚氨酯。采用N-异丙基丙烯酰胺单体对基于HTPB的聚氨酯进行紫外辐射改性，得到聚（N-异丙基丙烯酰胺）改性聚氨酯（PUDPANIPAAm）。利用基团贡献法计算了PUDPANIPAAm的内聚能（E(coh)）、摩尔体积（V）、溶解度参数（δ）、分子量（W(M)）、每克体积（V(g)）和密度（1/V(g)）。为了评估PUDPANIPAAm在伤口敷料和细胞片移植方面的应用，对PUDPANIPAAm膜的含水量、水蒸气透过率和气体渗透率进行了测定。在细胞培养中对这些膜的生物相容性、细胞黏附和增殖试验进行了研究。在初步研究中还评估了聚（N-异丙基丙烯酰胺）的热敏性对细胞脱离的影响。结果表明，这些PUDPANIPAAm膜具有热敏性。用聚（N-异丙基丙烯酰胺）对聚氨酯进行改性降低了水蒸气透过率和气体透过PUDPANIPAAm膜的渗透率。PUDPANIPAAm膜能够支持细胞黏附和生长。由于聚（N-异丙基丙烯酰胺）的热敏特性，从PUDPANIPAAm膜上脱离的相对细胞数比从聚苯乙烯培养皿上脱离的细胞数多。
