Marquele F D, Stracieri K M, Fonseca M J V, Freitas L A P
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.
Pharmazie. 2006 Apr;61(4):325-30.
The effect of spray drying on the chemical and biological properties of alcoholic extract of green propolis was investigated. The total polyphenol and flavonoid contents in spray-dried propolis extract were determined by the Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. The antioxidant activity of the dry extract was assessed by the membrane lipid peroxidation inhibition method, using quercetin as reference. The polyphenol content was shown to depend on the drying air outlet temperature and its square at the 0.5% significance level, while the flavonoid content depended only on the square of the outlet temperature at the 5% significance level. Polyphenol and flavonoid recovery after spray-drying ranged from 45.1 to 54.9% and 30.6 to 40.8%, respectively. The antioxidant activity of the spray-dried propolis was shown to be affected by the extract feed rate and air outlet temperature at a significance level of 0.1%. The spray-dried propolis extract showed significant antioxidant activity, with 50% lipid peroxidation inhibition at concentrations ranging from 2.5 to 5.0 microg/ml.
研究了喷雾干燥对绿蜂胶乙醇提取物化学和生物学性质的影响。分别采用福林-酚法和氯化铝比色法测定喷雾干燥蜂胶提取物中的总多酚和黄酮含量。以槲皮素为参比，采用膜脂质过氧化抑制法评估干提取物的抗氧化活性。结果表明，在0.5%显著水平下，多酚含量取决于干燥空气出口温度及其平方，而在5%显著水平下，黄酮含量仅取决于出口温度的平方。喷雾干燥后多酚和黄酮的回收率分别为45.1%至54.9%和30.6%至40.8%。在0.1%显著水平下，喷雾干燥蜂胶的抗氧化活性受提取物进料速率和空气出口温度的影响。喷雾干燥的蜂胶提取物表现出显著的抗氧化活性，在浓度为2.5至5.0微克/毫升时，脂质过氧化抑制率为50%。
