Department of Pharmaceutical Science, Health Sciences Center, Londrina State University (UEL), Avenue Robert Koch 60, 86038350 Londrina, PR, Brazil ; Department of Pharmaceutical Science, Faculty of Pharmaceutical Sciences of Ribeirao Preto (USP), Avenue Do Café s/n, 14040903 Ribeirao Preto, SP, Brazil.
Biomed Res Int. 2013;2013:340626. doi: 10.1155/2013/340626. Epub 2013 Aug 29.
This study evaluated the chemical properties (polyphenol and genistein contents) of soybean extracts obtained by biotransformation and dried by spray dryer at different conditions and their in vivo ability to inhibit 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced biochemical alterations in the skin of hairless mice. By comparing the obtained data with that of the well-known active soybean extract Isoflavin beta, we evaluated the influence of the fermentation and drying process in the extracts efficacy. The results demonstrated that inlet gas temperature and adjuvant concentration for the extract drying process have significantly affected the total polyphenol contents and, to a minor degree, the genistein contents. However, the effect of topical stimulus with TPA, an oxidative stress inducer, which caused significant depletion of reduced glutathione (GSH) and catalase, with increased levels of H2O2 and lipid peroxidation (MDA) in the skin of hairless mice, was significantly prevented by the soybean extracts treatment. These results indicate that the spray drying processing resulted in a product capable of limiting the oxidative stress with possible therapeutic applicability as an antioxidant in pharmaceutical forms.
本研究评估了通过生物转化获得的大豆提取物的化学性质（多酚和染料木黄酮含量），并在不同条件下通过喷雾干燥进行干燥，以及它们在体内抑制无毛小鼠皮肤中 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的生化变化的能力。通过将获得的数据与知名的活性大豆提取物异黄酮β进行比较，我们评估了发酵和干燥过程对提取物功效的影响。结果表明，进气温度和喷雾干燥用赋形剂浓度对总多酚含量有显著影响，对染料木黄酮含量的影响较小。然而，TPA（一种氧化应激诱导剂）的局部刺激作用导致无毛小鼠皮肤中还原型谷胱甘肽（GSH）和过氧化氢酶显著减少，H2O2 和脂质过氧化（MDA）水平增加，这一作用被大豆提取物的处理显著阻止。这些结果表明，喷雾干燥处理产生了一种能够限制氧化应激的产品，作为一种抗氧化剂，它可能具有治疗应用的潜力，以药物形式应用。
