Uchihi Rieko, Yamamoto Toshimichi, Yoshimoto Takashi, Inoue Chikako, Kishida Tetsuko, Yoshioka Naofumi, Katsumata Yoshinao
Department of Legal Medicine and Bioethics, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan.
Forensic Sci Int. 2007 Jul 4;169(2-3):255-9. doi: 10.1016/j.forsciint.2006.03.016. Epub 2006 May 2.
The genetic differences of the allele frequency distributions for six STR loci (D20S480, D6S2439, D6S1056, D9S1118, D4S2639, and D17S1290) among regions in Japan were examined using our recently designed hexaplex amplification and typing system, "Midi-6" newly named, to construct a database in the Japanese population. Genotypes at six loci were analyzed in 198, 200, 175, and 196 individuals from the area of Akita, Nagoya, Oita, and Okinawa, respectively, in Japan. The allele frequency distributions were significantly different (p<0.05) at from one to five loci among the four populations when compared pairwise. Significant differences were also observed at two or three loci between Oita- or Okinawa-Japanese and the "pooled" population (n=769), respectively. However, since F(ST) (theta) values were extremely low (<0.05), ranging from 0.0020 to 0.0118 for six loci, genetic differentiation within the pooled Japanese population was negligible. Therefore, it suggested that the data of the allele frequencies at six loci in the pooled population would be employed as the base of calculation for statistical probabilities.
使用我们最近设计的六重扩增和分型系统（新命名为“Midi-6”），检测了日本不同地区六个STR基因座（D20S480、D6S2439、D6S1056、D9S1118、D4S2639和D17S1290）等位基因频率分布的遗传差异，以构建日本人群的数据库。分别对来自日本秋田、名古屋、大分和冲绳地区的198、200、175和196名个体的六个基因座的基因型进行了分析。两两比较时，四个群体中一至五个基因座的等位基因频率分布存在显著差异（p<0.05）。大分或冲绳日本人与“合并”群体（n=769）之间在两个或三个基因座上也观察到显著差异。然而，由于F(ST)（theta）值极低（<0.05），六个基因座的F(ST)（theta）值范围为0.0020至0.0118，合并的日本人群体中的遗传分化可忽略不计。因此，建议将合并群体中六个基因座的等位基因频率数据用作统计概率计算的基础。
