Billaudel S, Aillet G, Tardivel P, Besse B, Sagot P, Le Guyader F, Mensier A, Lopes P
Laboratoire de Virologie, CHRU Nantes, Hôtel-Dieu.
J Gynecol Obstet Biol Reprod (Paris). 1991;20(7):941-5.
The typing fo Human Papillomavirus (HPV) was carried out in cervical lesions in order to decide on the best therapy to carry out in mild and medium dysplasias of the cervix. 131 patients who had an iodo-negative zone or a smear suggesting an HPV infection had microbiopsies carried out under colposcopic control. Every lesion had two biopsies carried out side by side to study the histopathology and the virology. Dysplastic lesions were found in 93 biopsies. A search for the DNA of HPV 6a, 16 and 18 was carried out using a Southern blot technique with 32P following the method of Random multipriming. Hybridization was carried out in strict and non-strict conditions. In 43 cases out of 93 dysplasias a virus was found: 2 HPV 6a, 14 HPV 16, 1 HPV 18 and 26 X HPV's (not typed). The number of HPV's that could be detected increased according to the severity of the dysplasia which was also found in frequency of type 16. Where the biopsies showed no dysplasia type 16 was found in four out of seven viruses. In this study electrophoretic profiles for type 16 were found in four cases suggesting that they were integrated into the chromosome of the cell. The low percentage of HPV found in mild or moderate dysplasias (4 type 16 out of 61 biopsies) shows that at present this test can not be used as an aid in choosing treatment.
对宫颈病变进行人乳头瘤病毒（HPV）分型，以便确定针对宫颈轻度和中度发育异常的最佳治疗方法。131例碘阴性区或涂片提示HPV感染的患者在阴道镜检查控制下进行了微生物活检。每个病变部位并行进行两次活检，以研究组织病理学和病毒学。在93次活检中发现了发育异常病变。采用随机多引物法，用³²P进行Southern印迹技术检测HPV 6a、16和18的DNA。在严格和非严格条件下进行杂交。在93例发育异常病例中，有43例发现病毒：2例HPV 6a、14例HPV 16、1例HPV 18和26例未分型的HPV。可检测到的HPV数量随着发育异常的严重程度增加而增加，HPV 16型的频率也如此。活检未显示发育异常的病例中，7例病毒中有4例发现HPV 16型。在本研究中，4例发现16型的电泳图谱，提示它们已整合到细胞染色体中。在轻度或中度发育异常中发现的HPV比例较低（61次活检中有4例16型），表明目前该检测不能用于辅助选择治疗方法。
