Yanaga Hiroko, Yanaga Katsu, Imai Keisuke, Koga Mika, Soejima Chie, Ohmori Kitaro
Yanaga Clinic and Tissue Culture Laboratory, Osaka City General Hospital, Fukuoka, Japan.
Plast Reconstr Surg. 2006 May;117(6):2019-30; discussion 2031-2. doi: 10.1097/01.prs.0000210662.12267.de.
The repair of a craniofacial or nose deformity requires a large volume of reconstructive material. A conventional cartilage graft does not provide a sufficient volume of reconstructive material. Therefore, augmentation of the facial form to the defect shape is quite difficult. The authors developed a new treatment method that provides a sufficiently large volume of reconstructive material and enables an easier reconstruction of the original shape.
Ages of the patients ranged between 9 and 63 years. Approximately 1 cm of auricular cartilage was collected from the auricular concha. Isolated chondrocytes were cultured with autologous serum that accelerates cell proliferation. The cells were subcultured and formed a gel-form mass. This mass, together with autologous serum, was grafted (injected) on the periosteum and into the subcutaneous pocket. The volume of grafted cultured chondrocytes ranged from 1.7 to 40 cc (1 to 5 x 10(7) cells/cc). The lesion changed from soft gel form into hard cartilage tissues within 2 to 3 weeks and stabilized.
Excellent or good satisfactory results were obtained in all patients and have been maintained for periods ranging from 3 to 34 months. No patient experienced absorption of cultured chondrocytes. Biopsy of the newly formed tissues showed that it was an elastic cartilage derived from the original tissue.
A small number of chondrocytes obtained from a 1-cm auricular cartilage are successfully cultured into a large number of cells in a gel form. Those autologous auricular chondrocytes in a gel form allow for the repair of complicated shapes of the defect area. This technique is applicable to various treatments for craniofacial or nose deformity.
颅面或鼻部畸形的修复需要大量的重建材料。传统的软骨移植无法提供足够量的重建材料。因此，使面部形态与缺损形状相匹配相当困难。作者开发了一种新的治疗方法，该方法可提供足够大量的重建材料，并能更轻松地重建原始形状。
患者年龄在9岁至63岁之间。从耳甲收集约1厘米的耳廓软骨。将分离的软骨细胞与可加速细胞增殖的自体血清一起培养。细胞进行传代培养并形成凝胶状团块。将该团块与自体血清一起移植（注射）到骨膜上和皮下囊袋中。移植的培养软骨细胞体积为1.7至40立方厘米（1至5×10⁷个细胞/立方厘米）。病变在2至3周内从软凝胶形式转变为硬软骨组织并稳定下来。
所有患者均获得了优异或良好的满意效果，并持续了3至34个月。没有患者出现培养软骨细胞吸收的情况。对新形成组织的活检表明，它是源自原始组织的弹性软骨。
从1厘米耳廓软骨中获取的少量软骨细胞成功培养成大量凝胶形式的细胞。这些凝胶形式的自体耳廓软骨细胞可用于修复缺损区域的复杂形状。该技术适用于颅面或鼻部畸形的各种治疗。
