Nayernia Karim, Lee Jae Ho, Drusenheimer Nadja, Nolte Jessica, Wulf Gerald, Dressel Ralf, Gromoll Jörg, Engel Wolfgang
Institute of Human Genetics, University of Göttingen, Göttingen, Germany.
Lab Invest. 2006 Jul;86(7):654-63. doi: 10.1038/labinvest.3700429. Epub 2006 May 1.
Recent studies have demonstrated that somatic stem cells have a more flexible potential than expected, whether put into tissue or cultured under different conditions. Bone marrow (BM)-derived stem cells can transdifferentiate into multilineage cells, such as muscle of mesoderm, lung and liver of endoderm, and brain and skin of ectoderm origin. Here we show that BM stem cells are able to transdifferentiate into male germ cells. For derivation of male germ cells from adult BM stem (BMS) cells, we used the Stra8-enhanced green fluoresence protein (EGFP) transgenic mouse line expressing EGFP specifically in male germ cells. BMS cell-derived germ cells expressed the known molecular markers of primordial germ cells, such as fragilis, stella, Rnf17, Mvh and Oct4; as well as molecular markers of spermatogonial stem cells and spermatogonia including Rbm, c-Kit, Tex18, Stra8, Piwil2, Dazl, Hsp90alpha, beta1- and alpha6-integrins. Our ability to derive male germ cells from BMS cells reveals novel aspects of germ cell development and opens the possibilities for use of these cells in reproductive medicine.
最近的研究表明，无论将体干细胞置于组织中还是在不同条件下培养，其潜能都比预期的更为灵活。骨髓（BM）来源的干细胞能够转分化为多谱系细胞，如中胚层来源的肌肉、内胚层来源的肺和肝脏以及外胚层来源的脑和皮肤。在此，我们展示了BM干细胞能够转分化为雄性生殖细胞。为了从成年BM干细胞（BMS细胞）中获得雄性生殖细胞，我们使用了Stra8增强型绿色荧光蛋白（EGFP）转基因小鼠品系，该品系在雄性生殖细胞中特异性表达EGFP。BMS细胞来源的生殖细胞表达了已知的原始生殖细胞分子标志物，如fragilis、stella、Rnf17、Mvh和Oct4；以及精原干细胞和精原细胞的分子标志物，包括Rbm、c-Kit、Tex18、Stra8、Piwil2、Dazl、Hsp90alpha、β1-和α6-整合素。我们从BMS细胞中获得雄性生殖细胞的能力揭示了生殖细胞发育的新方面，并为这些细胞在生殖医学中的应用开辟了可能性。
