Lillegard Joseph B, Sim Robert B, Thorkildson Peter, Gates Marcellene A, Kozel Thomas R
Department of Microbiology and Immunology, University of Nevada School of Medicine, and Cell, Reno, 89557, USA.
J Infect Dis. 2006 Jun 1;193(11):1589-97. doi: 10.1086/503804. Epub 2006 Apr 25.
Mannan-binding lectin (MBL) is a component of the innate immune system. The goal of the present study was to evaluate binding of MBL to Candida albicans in vitro and in vivo and to assess the impact of MBL treatment on host resistance. The results showed a variable and often discontinuous pattern of binding to individual yeast cells. MBL bound to cells grown at 37 degrees C but not to cells grown at 23 degrees C. The putative MBL ligand was constitutively present on yeast cells grown at 23 degrees C, but the ligand was masked on such cells, such that MBL could not bind. C. albicans yeasts and hyphae in infected tissue bound MBL. Finally, parenteral administration of MBL increased resistance of mice to hematogenously disseminated candidiasis. These results suggest that MBL is an important component of innate resistance to candidiasis and that MBL therapy may be a means to prevent disseminated candidiasis in high-risk patients.
甘露聚糖结合凝集素（MBL）是固有免疫系统的一个组成部分。本研究的目的是评估MBL在体外和体内与白色念珠菌的结合情况，并评估MBL治疗对宿主抵抗力的影响。结果显示，MBL与单个酵母细胞的结合模式各不相同，且往往是不连续的。MBL能与在37℃培养的细胞结合，但不能与在23℃培养的细胞结合。假定的MBL配体在23℃培养的酵母细胞上组成性存在，但该配体在这类细胞上被掩盖，使得MBL无法结合。感染组织中的白色念珠菌酵母和菌丝结合了MBL。最后，经肠外给予MBL可增强小鼠对血源性播散性念珠菌病的抵抗力。这些结果表明，MBL是对念珠菌病固有抵抗力的一个重要组成部分，并且MBL治疗可能是预防高危患者发生播散性念珠菌病的一种手段。
