Clapp N K, Henke M A, Hansard R M, Walsh R E, Widomski D L, Anglin C P, Fretland D J, Gaginella T S
Marmoset Research Center, Oak Ridge Associated Universities, TN 37831.
Agents Actions. 1991 Sep;34(1-2):178-80. doi: 10.1007/BF01993271.
Spontaneous colitis in CTT's presents cytological characteristics similar to chronic ulcerative colitis in humans, e.g. inflammatory cell infiltrate and crypt abscesses. To better characterize CTT colitis as a potential model for human inflammatory bowel disease (IBD), inflammatory mediators identified in colonic tissue of human IBD patients and/or experimental colitis models were assayed. Inflammatory mediator changes in plasma and colon from tamarins with acute (n = 10) and chronic (n = 10) colitis (by mucosal biopsy) were assayed by RIAs. Similar inflammatory mediators were found in the CTT's with acute colitis. In the plasma, PAF and PGE2 levels were lower in acute colitis CTT's, no LTB4 was detected, and histamine levels were not different from chronic colitic animals. In the colon, myeloperoxidase and interleukin-1 beta were significantly higher in acute colitis, PGE2 and LTB4 were higher but not significantly, and PAF was not different from chronic CTT's. These data suggest that a combination of events are occurring in the pathogenesis of tamarin colitis that involves some of the same mediators that are found in the human disease and in other experimental models. The importance of these findings to human IBD remains for further investigation; however, the spontaneous primate model offers an exciting approximation of the disease development and merits further investigation for understanding the pathogenesis of human IBD as well as to aid in development of targeted therapeutics.
普通棉耳狨猴的自发性结肠炎呈现出与人类慢性溃疡性结肠炎相似的细胞学特征，例如炎性细胞浸润和隐窝脓肿。为了更好地将普通棉耳狨猴结肠炎表征为人类炎症性肠病（IBD）的潜在模型，对在人类IBD患者结肠组织和/或实验性结肠炎模型中鉴定出的炎性介质进行了检测。通过放射免疫分析法检测了患有急性（n = 10）和慢性（n = 10）结肠炎（通过黏膜活检）的绢毛猴血浆和结肠中的炎性介质变化。在患有急性结肠炎的普通棉耳狨猴中发现了类似的炎性介质。在血浆中，急性结肠炎普通棉耳狨猴的血小板活化因子（PAF）和前列腺素E2（PGE2）水平较低，未检测到白三烯B4（LTB4），组胺水平与慢性结肠炎动物无差异。在结肠中，急性结肠炎时髓过氧化物酶和白细胞介素-1β显著升高，PGE2和LTB4升高但不显著，PAF与慢性普通棉耳狨猴无差异。这些数据表明，绢毛猴结肠炎发病机制中发生的一系列事件涉及一些与人类疾病和其他实验模型中相同的介质。这些发现对人类IBD的重要性仍有待进一步研究；然而，这种自发性灵长类动物模型为疾病发展提供了一个令人兴奋的近似模型，对于理解人类IBD的发病机制以及辅助开发靶向治疗方法具有进一步研究的价值。
