Perez-Prat E, Narasimhan M L, Binzel M L, Botella M A, Chen Z, Valpuesta V, Bressan R A, Hasegawa P M
Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907-1165.
Plant Physiol. 1992 Nov;100(3):1471-8. doi: 10.1104/pp.100.3.1471.
A cDNA clone was isolated that encodes the partial sequence of a putative endoplasmic reticulum Ca(2+)-ATPase of tobacco. The 1.497-kb insert had an open reading frame of 1.149 kb. The deduced peptide had the greatest homology to the endoplasmic reticulum Ca(2+)-ATPases of Drosophila and Artemia, followed by the mammalian and avian enzymes (SERCA2 and 3). The cDNA insert hybridized to a single mRNA of 4.4 kb from tobacco cultured cells or plant tissues. The level of this transcript was induced about 2-fold by NaCl shock in 428 mm NaCl-deadapted tobacco cells that were maintained in medium without salt, but not in unadapted cells. The level of this transcript was 3- to 4-fold higher in 428 mm NaCl-adapted cells growing in salt than in unadapted cells growing without salt.
分离出一个编码烟草假定内质网Ca(2+) - ATP酶部分序列的cDNA克隆。1.497 kb的插入片段有一个1.149 kb的开放阅读框。推导的肽与果蝇和卤虫的内质网Ca(2+) - ATP酶有最大的同源性，其次是哺乳动物和鸟类的酶（SERCA2和3）。该cDNA插入片段与来自烟草培养细胞或植物组织的4.4 kb单一mRNA杂交。在无盐培养基中维持的428 mM NaCl去适应烟草细胞中，该转录本的水平在NaCl冲击下诱导约2倍，但在未适应细胞中则不然。在盐中生长的428 mM NaCl适应细胞中，该转录本的水平比在无盐条件下生长的未适应细胞高3至4倍。
