• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过在电通透处理后的细胞之间建立接触，可实现培养中的哺乳动物细胞融合。

Fusion of mammalian cells in culture is obtained by creating the contact between cells after their electropermeabilization.

作者信息

Teissie J, Rols M P

出版信息

Biochem Biophys Res Commun. 1986 Oct 15;140(1):258-66. doi: 10.1016/0006-291x(86)91084-3.

DOI:10.1016/0006-291x(86)91084-3
PMID:3778446
Abstract

Electropermeabilization of chinese hamster ovary cells (CHO) is a long lived process but is nevertheless fully reversible. Electrofusion can be obtained by centrifuging the cells several minutes after the pulsation and then by incubating the pellet. These results show that fusion is not directly created by the field but is due to the existence of a peculiar state of the plasma membrane. The permeabilized patches are local on the cell surface and do not spread or diffuse on it.

摘要

中国仓鼠卵巢细胞（CHO）的电通透化是一个持续时间较长的过程，但仍然是完全可逆的。在脉冲后对细胞进行几分钟的离心，然后对沉淀进行孵育，即可实现电融合。这些结果表明，融合并非由电场直接产生，而是由于质膜存在一种特殊状态。通透化的斑块位于细胞表面局部，不会在细胞表面扩散。

相似文献

1
Fusion of mammalian cells in culture is obtained by creating the contact between cells after their electropermeabilization.通过在电通透处理后的细胞之间建立接触，可实现培养中的哺乳动物细胞融合。
Biochem Biophys Res Commun. 1986 Oct 15;140(1):258-66. doi: 10.1016/0006-291x(86)91084-3.
2
Induction of a long-lived fusogenic state in viable plant protoplasts permeabilized by electric fields.在经电场通透处理的活植物原生质体中诱导产生长寿的融合状态。
Biochim Biophys Acta. 1990 May 9;1024(1):203-7. doi: 10.1016/0005-2736(90)90227-f.
3
Reversible plasma membrane ultrastructural changes correlated with electropermeabilization in Chinese hamster ovary cells.中国仓鼠卵巢细胞中与电通透化相关的可逆性质膜超微结构变化
Biochim Biophys Acta. 1988 Apr 7;939(2):247-59. doi: 10.1016/0005-2736(88)90068-5.
4
Pulse-first heterofusion of cells by electric field pulses and associated loading of macromolecules into mammalian cells.通过电场脉冲实现细胞的脉冲式异质融合以及将大分子物质导入哺乳动物细胞。
Biotechniques. 1994 Oct;17(4):762-4, 766-9.
5
Modulation of electrically induced permeabilization and fusion of Chinese hamster ovary cells by osmotic pressure.渗透压对中国仓鼠卵巢细胞电诱导通透性和融合的调节作用
Biochemistry. 1990 May 15;29(19):4561-7. doi: 10.1021/bi00471a009.
6
Electrofusion between heterogeneous-sized mammalian cells in a pellet: potential applications in drug delivery and hybridoma formation.沉淀中不同大小哺乳动物细胞间的电融合：在药物递送和杂交瘤形成中的潜在应用。
Biophys J. 1996 Jul;71(1):479-86. doi: 10.1016/S0006-3495(96)79249-4.
7
Ionic-strength modulation of electrically induced permeabilization and associated fusion of mammalian cells.电诱导哺乳动物细胞透化及相关融合的离子强度调节
Eur J Biochem. 1989 Jan 15;179(1):109-15. doi: 10.1111/j.1432-1033.1989.tb14527.x.
8
Electrofusion of Chinese hamster ovary cells after ethanol incubation.乙醇孵育后中国仓鼠卵巢细胞的电融合
Biochim Biophys Acta. 1985 Oct 24;820(1):58-62. doi: 10.1016/0005-2736(85)90215-9.
9
Hybridization frequencies of different mammalian cell types by electrofusion.通过电融合对不同哺乳动物细胞类型的杂交频率
Gen Physiol Biophys. 1990 Oct;9(5):489-99.
10
Experimental evidence for the involvement of the cytoskeleton in mammalian cell electropermeabilization.细胞骨架参与哺乳动物细胞电穿孔的实验证据。
Biochim Biophys Acta. 1992 Oct 19;1111(1):45-50. doi: 10.1016/0005-2736(92)90272-n.

引用本文的文献

1
Cell electrofusion based on nanosecond/microsecond pulsed electric fields.基于纳秒/微秒脉宽电场的细胞电融合。
PLoS One. 2018 May 24;13(5):e0197167. doi: 10.1371/journal.pone.0197167. eCollection 2018.
2
Exploring the Applicability of Nano-Poration for Remote Control in Smart Drug Delivery Systems.探索纳米孔技术在智能药物递送系统中用于远程控制的适用性。
J Membr Biol. 2017 Feb;250(1):31-40. doi: 10.1007/s00232-016-9922-1. Epub 2016 Aug 25.
3
The Influence of Vesicle Shape and Medium Conductivity on Possible Electrofusion under a Pulsed Electric Field.
囊泡形状和介质电导率对脉冲电场下可能的电融合的影响。
PLoS One. 2016 Jul 8;11(7):e0158739. doi: 10.1371/journal.pone.0158739. eCollection 2016.
4
Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies.利用人杂交瘤技术分离人单克隆抗体。
Microbiol Spectr. 2015 Feb;3(1):AID-0027-2014. doi: 10.1128/microbiolspec.AID-0027-2014.
5
Cell electrofusion using nanosecond electric pulses.使用纳秒电脉冲进行细胞电融合。
Sci Rep. 2013 Nov 29;3:3382. doi: 10.1038/srep03382.
6
Destabilizing giant vesicles with electric fields: an overview of current applications.用电场使巨型囊泡不稳定：当前应用概述。
J Membr Biol. 2012 Sep;245(9):555-64. doi: 10.1007/s00232-012-9467-x. Epub 2012 Aug 5.
7
The systematic study of the electroporation and electrofusion of B16-F1 and CHO cells in isotonic and hypotonic buffer.在等渗和低渗缓冲液中对 B16-F1 和 CHO 细胞的电穿孔和电融合进行系统研究。
J Membr Biol. 2012 Sep;245(9):583-90. doi: 10.1007/s00232-012-9470-2. Epub 2012 Jul 29.
8
Cell-cell electrofusion: optimization of electric field amplitude and hypotonic treatment for mouse melanoma (B16-F1) and Chinese Hamster ovary (CHO) cells.细胞-细胞电融合：优化电场幅度和低渗处理对小鼠黑色素瘤（B16-F1）和中国仓鼠卵巢（CHO）细胞的影响。
J Membr Biol. 2010 Jul;236(1):107-16. doi: 10.1007/s00232-010-9272-3. Epub 2010 Jul 14.
9
Recent advances in the study of human antibody responses to influenza virus using optimized human hybridoma approaches.优化人杂交瘤技术研究人类针对流感病毒的抗体反应的最新进展。
Vaccine. 2009 Dec 30;27 Suppl 6:G47-51. doi: 10.1016/j.vaccine.2009.10.124.
10
Electrically induced protoplast fusion using pulse electric fields for dielectrophoresis.电脉冲诱导原生质体融合用于电泳。
Plant Physiol. 1992 Dec;100(4):2008-12. doi: 10.1104/pp.100.4.2008.