光照和环境溶质对豌豆叶片完整叶绿体分离的核编码基质蛋白分解代谢的影响。

Effects of light and external solutes on the catabolism of nuclear-encoded stromal proteins in intact chloroplasts isolated from pea leaves.

机构信息

Institute of Plant Physiology, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland.

出版信息

Plant Physiol. 1992 Dec;100(4):2100-5. doi: 10.1104/pp.100.4.2100.

DOI:10.1104/pp.100.4.2100

PMID:16653246

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1075913/

Abstract

The catabolism of nuclear-encoded stromal proteins was investigated in intact chloroplasts isolated mechanically from pea (Pisum sativum) leaves. Glutamine synthetase, phosphoribulokinase, and nitrite reductase (quantified by immunoblotting) were more rapidly degraded in the light than in the dark. Furthermore, the degradation rates depended on exogenously supplied metabolites. For example, 2-oxoglutarate accelerated the catabolism of all three enzymes in chloroplasts incubated in the light, whereas oxaloacetate stabilized glutamine synthetase and at the same time destabilized the other two enzymes.

摘要

我们研究了从豌豆（Pisum sativum）叶片中机械分离的完整叶绿体中核编码基质蛋白的分解代谢。与黑暗相比，在光照下，谷氨酰胺合成酶、磷酸核糖激酶和亚硝酸盐还原酶（通过免疫印迹定量）更快降解。此外，降解速率取决于外源性代谢物。例如，在光照下孵育的叶绿体中，2-氧戊二酸加速了这三种酶的分解代谢，而草酰乙酸稳定了谷氨酰胺合成酶，同时使其他两种酶失稳。