Bao Xuefeng, Lin Guolin, Dong Xuan, Ma Xinyu, Han Xiaori, Zhang Hui
College of Land and Environment Shenyang Agricultural University Shenyang China.
Food Sci Nutr. 2021 Jan 19;9(3):1471-1479. doi: 10.1002/fsn3.2117. eCollection 2021 Mar.
This study aimed to investigate the effects of exogenous copper and ethylene on resveratrol biosynthesis in peanut buds. In this study, different concentrations of copper sulfate and ethephon were used to induce peanut bud roots. Resveratrol content was determined using high-performance liquid chromatography (HPLC). The interaction effects of the two inducers were analyzed using regression and response surface methodology. Results showed that resveratrol biosynthesis increased with the increase in copper sulfate concentration. Resveratrol synthesis showed an increasing trend when the concentration of ethephon was from 5 to 7 mmol/L. The resveratrol content reached a maximum value of 7.7 μg/g when the concentration of ethephon was 7 mmol/L and then decreased with an increase in ethephon. Response surface analysis revealed that the combination of 0.1 mmol/L copper sulfate and 5 mmol/L ethephon was effective and resulted in the induction of resveratrol biosynthesis in peanut buds.
本研究旨在探讨外源铜和乙烯对花生芽中白藜芦醇生物合成的影响。在本研究中，使用不同浓度的硫酸铜和乙烯利诱导花生芽根。采用高效液相色谱法（HPLC）测定白藜芦醇含量。运用回归和响应面分析法分析两种诱导剂的交互作用。结果表明，白藜芦醇的生物合成随着硫酸铜浓度的增加而增加。当乙烯利浓度在5至7 mmol/L时，白藜芦醇合成呈上升趋势。当乙烯利浓度为7 mmol/L时，白藜芦醇含量达到最大值7.7 μg/g，随后随着乙烯利浓度的增加而降低。响应面分析表明，0.1 mmol/L硫酸铜和5 mmol/L乙烯利的组合是有效的，可诱导花生芽中白藜芦醇的生物合成。
