Hoson T, Masuda Y, Sone Y, Misaki A
Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan.
Plant Physiol. 1991 Jun;96(2):551-7. doi: 10.1104/pp.96.2.551.
Polyclonal antibodies were raised in rabbits against isoprimeverose (Xyl(1)Glc(1)), xyloglucan heptasaccharides (Xyl(3)Glc(4)), and octasaccharides (Gal(1)Xyl(3)Glc(4)). Antibodies specific for hepta- and octasaccharides suppressed auxin-induced elongation of epicotyl segments of azuki bean (Vigna angularis Ohwi and Ohashi cv Takara). These antibodies also inhibited auxin-induced cell wall loosening (decrease in the minimum stress-relaxation time and the relaxation rate of the cell walls) of azuki segments. However, none of the antibodies influenced auxin-induced elongation or cell wall loosening of coleoptile segments of oat (Avena sativa L. cv Victory). Auxin caused a decrease in molecular mass of xyloglucans in the cell walls of azuki epicotyls and oat coleoptiles. The antibodies inhibited such a change in molecular mass of xyloglucans in both species. Preimmune serum exhibited little or no inhibitory effect on auxin-induced elongation, cell wall loosening, or breakdown of xyloglucans. The results support the view that the breakdown of xyloglucans is associated with the cell wall loosening responsible for auxin-induced elongation in dicotyledons. The view does not appear to be applicable to poaceae, because the inhibition of xyloglucan breakdown by the antibodies did not influence auxin-induced elongation or cell wall loosening of oat coleoptiles.
针对异麦芽糖（木糖(1)葡萄糖(1)）、木葡聚糖七糖（木糖(3)葡萄糖(4)）和八糖（半乳糖(1)木糖(3)葡萄糖(4)）在兔子体内制备了多克隆抗体。对七糖和八糖具有特异性的抗体抑制了生长素诱导的赤小豆（Vigna angularis Ohwi and Ohashi cv Takara）上胚轴切段的伸长。这些抗体也抑制了生长素诱导的赤小豆切段细胞壁松弛（细胞壁最小应力松弛时间和松弛速率降低）。然而，这些抗体均未影响生长素诱导的燕麦（Avena sativa L. cv Victory）胚芽鞘切段的伸长或细胞壁松弛。生长素导致赤小豆上胚轴和燕麦胚芽鞘细胞壁中的木葡聚糖分子量降低。抗体抑制了这两个物种中木葡聚糖分子量的这种变化。免疫前血清对生长素诱导的伸长、细胞壁松弛或木葡聚糖降解几乎没有抑制作用。结果支持了木葡聚糖降解与双子叶植物中生长素诱导伸长所涉及的细胞壁松弛相关的观点。该观点似乎不适用于禾本科植物，因为抗体对木葡聚糖降解的抑制并未影响燕麦胚芽鞘切段的生长素诱导伸长或细胞壁松弛。
