The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
Plant Physiol. 1968 Apr;43(4):635-40. doi: 10.1104/pp.43.4.635.
The endogenous respiration rate of unstarved cultured cells of Acer pseudo-platanus L. is markedly stimulated by 2,4-dinitrophenol. Pyruvate is also stimulatory but to a lesser degree than dinitrophenol. Exogenously supplied sugars cause no short-term stimulation. Pyruvate does not enhance the elevated rate of O(2) uptake in the presence of dinitrophenol but does cause additional CO(2) evolution. The endogenous concentration of pyruvate is elevated in the presence of dinitrophenol. These observations suggest that the rate of O(2) uptake by the unstarved intact cells is limited by the rate of glycolysis and that rate of glycolysis is regulated by the intracellular concentration of adenine nucleotides or inorganic phosphate. Dinitrophenol stimulation of endogenous respiration is due in part to an indirect acceleration of glycolysis but also to a more direct facilitation of oxidation in the presence of excess mitochondrial substrate.
未饥饿培养的 Acer pseudo-platanus L. 细胞的内呼吸率明显被 2,4-二硝基苯酚刺激。丙酮酸也有刺激作用，但程度低于二硝基苯酚。外源提供的糖不会引起短期刺激。丙酮酸不会增强存在二硝基苯酚时 O(2)摄取的升高速率，但会导致额外的 CO(2)释放。在二硝基苯酚存在下，丙酮酸的内源性浓度升高。这些观察结果表明，未饥饿完整细胞的 O(2)摄取速率受糖酵解速率的限制，而糖酵解速率受细胞内腺嘌呤核苷酸或无机磷酸盐浓度的调节。二硝基苯酚对内呼吸的刺激部分归因于糖酵解的间接加速，部分归因于在线粒体底物过剩的情况下氧化的更直接促进。
