盐度对豌豆根中苹果酸脱氢酶的影响。
The effect of salinity on the malic dehydrogenase of pea roots.
机构信息
Department of Botany, The Hebrew University of Jerusalem, Israel.
出版信息
Plant Physiol. 1969 Jul;44(7):1031-4. doi: 10.1104/pp.44.7.1031.
Effect of salinity on malate dehydrogenase activity was studied.Pea root tips contain 2 different malate dehydrogenases. One is located in the particulate, mitochondrial fraction, the other in the soluble, cytoplasmic fraction. Both can act when coupled with either NAD or NADP.Growing plants in Na(2)SO(4) salinated medium did not affect the pattern of the malate dehydrogenases in the root tips. Growing plants in NaCl salinated media resulted in the appearance of a new, third isoenzyme. This new isoenzyme was located in the cytoplasmic fraction.Salinity of both types, when present in growth medium, induced increases in the NADP coupled activity of the mitochondrial malate dehydrogenase. The NAD coupled activity, however, was depressed except in the cytoplasmic fraction of plants grown in media salinated with NaCl to 1 atmosphere. Addition of either of the salts to assay media of enzymes, isolated from plants grown in non salinated substrate, did not have any significant effect.
研究了盐度对苹果酸脱氢酶活性的影响。豌豆根尖含有 2 种不同的苹果酸脱氢酶。一种位于颗粒状、线粒体部分，另一种位于可溶性、细胞质部分。当与 NAD 或 NADP 偶联时，两者都可以起作用。在含有 Na2SO4 的盐渍培养基中生长的植物不会影响根尖中苹果酸脱氢酶的模式。在含有 NaCl 的盐渍培养基中生长的植物会产生一种新的、第三种同工酶。这种新的同工酶位于细胞质部分。当生长介质中存在这两种类型的盐度时，会诱导线粒体苹果酸脱氢酶与 NADP 偶联的活性增加。然而，与 NAD 偶联的活性除了在 NaCl 盐渍介质中生长的植物的细胞质部分外都受到抑制，NaCl 盐度为 1 大气压。将这两种盐中的任何一种添加到从非盐渍底物中生长的植物中分离的酶的测定介质中，没有任何显著影响。
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