Department of Biological Sciences, Purdue University, Lafayette, Indiana.
Plant Physiol. 1966 Apr;41(4):713-7. doi: 10.1104/pp.41.4.713.
The major conclusion drawn was that malate generated in corn roots during a 15-minute period of CO(2) fixation and malate introduced into the tissue during a similar period from the bathing medium share a common extramitochondrial compartment, the cytoplasmic pool. The utilization of these 2 forms of malate is normally much slower than that of malate generated in the mitochondria by the tricarboxylic acid cycle. By lowering the pH of the medium or treating the tissue with malonate or 2,4-dinitrophenol, similar increases in the rates of utilization of both forms of cytoplasmic malate were brought about. Changes in (A) the demand for acetyl acceptors in the mitochondria and (B) mitochondrial permeability were invoked to account for the increased utilization of the cytoplasmic malate under the various experimental treatments.
主要结论是，在 CO(2)固定的 15 分钟期间在玉米根中生成的苹果酸和在类似时间段内从浴液中引入组织的苹果酸共享一个共同的细胞外线粒体隔室，即细胞质池。这些 2 种形式的苹果酸的利用速度通常比三羧酸循环在线粒体中生成的苹果酸慢得多。通过降低介质的 pH 值或用丙二酸盐或 2,4-二硝基苯酚处理组织，可以使这两种形式的细胞质苹果酸的利用速率相似地增加。通过改变（A）线粒体中乙酰受体的需求和（B）线粒体通透性，来解释在各种实验处理下细胞质苹果酸利用率的增加。
