Correll D L, Edwards J L
Radiation Biology Laboratory, Smithsonian Institution, Rockville, Maryland 20852.
Plant Physiol. 1970 Jan;45(1):81-5. doi: 10.1104/pp.45.1.81.
Initial extracts from etiolated plants contained two aggregates of phytochrome. A major fraction was almost excluded by Sephadex G-200 and was within the fractionation range of Sepharose 4 B. A minor fraction was within the fractionation range of Sephadex G-200. Modifications of a previous isolation procedure which allowed retention of this aggregation state are reported. With respect to gel filtration, the major fraction of phytochrome from oat and rye seedlings was identical. The aggregates of rye and oat phytochrome were also separated by diethylaminoethyl cellulose chromatography.
黄化植物的初始提取物含有两种光敏色素聚集体。主要部分几乎被葡聚糖G - 200排除在外，处于琼脂糖4B的分级分离范围内。次要部分处于葡聚糖G - 200的分级分离范围内。本文报道了对先前分离程序的改进，该改进可使这种聚集状态得以保留。关于凝胶过滤，燕麦和黑麦幼苗中的主要光敏色素部分是相同的。黑麦和燕麦光敏色素的聚集体也通过二乙氨基乙基纤维素色谱法分离。
