Department of Biology, Vanderbilt University, Nashville, Tennessee 37235.
Plant Physiol. 1979 Aug;64(2):332-6. doi: 10.1104/pp.64.2.332.
We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, isoelectric focusing, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We have also used the immunoaffinity technique to purify phytochrome from crude oat extracts, and from brushite-purified pea (Pisum sativum L., cv. Alaska) and rye (Secale cereale L., cv. Balbo) preparations.
我们开发了一种光敏色素免疫亲和纯化程序，当起始材料为鸟粪石纯化制备物时，在 2 小时内可得到纯度大于 98%的未降解的燕麦（Avena sativa L.，cv. Garry）光敏色素。免疫亲和纯化的光敏色素，除了更高的纯度外，通过凝胶排阻层析、等电聚焦和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳与常规纯化的光敏色素无法区分。我们还使用免疫亲和技术从粗燕麦提取物以及鸟粪石纯化的豌豆（Pisum sativum L.，cv. Alaska）和黑麦（Secale cereale L.，cv. Balbo）制剂中纯化光敏色素。
