Department of Chemistry, University of Colorado, Boulder, Colorado 80302.
Plant Physiol. 1970 Jun;45(6):675-8. doi: 10.1104/pp.45.6.675.
Several glycosidases have been isolated from suspensioncultured sycamore (Acer pseudoplatanus) cells. These include an alpha-galactosidase, an alpha-mannosidase, a beta-N-acetyl-glucosaminidase, a beta-glucosidase, and two beta-galactosidases. The pH optimum of each of these enzymes was determined. The pH optima, together with inhibition studies, suggest that each observed glycosidase activity represents a separate enzyme. Three of these enzymes, beta-glucosidase, alpha-galactosidase, and one of the beta-galactosidases, have been shown to be associated with the cell surface. The enzyme activities associated with the cell surface were shown to possess the ability to degrade to a limited extent isolated sycamore cell walls. It was found that the activities of beta-glucosidase and of one of the beta-galactosidases increase as the cells go through a period of growth and decrease as cell growth ceases.
已经从悬浮培养的悬铃木（Acer pseudoplatanus）细胞中分离出几种糖苷酶。其中包括α-半乳糖苷酶、α-甘露糖苷酶、β-N-乙酰葡萄糖胺酶、β-葡萄糖苷酶和两种β-半乳糖苷酶。确定了每种酶的最适 pH 值。最适 pH 值以及抑制研究表明，每种观察到的糖苷酶活性都代表一种单独的酶。其中三种酶，β-葡萄糖苷酶、α-半乳糖苷酶和一种β-半乳糖苷酶，已被证明与细胞表面有关。与细胞表面有关的酶活性被证明具有有限程度降解分离悬铃木细胞壁的能力。结果发现，β-葡萄糖苷酶和一种β-半乳糖苷酶的活性随着细胞生长而增加，随着细胞生长停止而减少。
