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悬浮培养的枫杨-槭细胞中富含羟脯氨酸成分的合成和运输。

Synthesis and Transport of Hydroxyproline-rich Components in Suspension Cultures of Sycamore-Maple Cells.

机构信息

Michigan State University, Michigan State University-Atomic Energy Commission Plant Research Laboratory, East Lansing, Michigan 48823.

出版信息

Plant Physiol. 1970 Dec;46(6):831-8. doi: 10.1104/pp.46.6.831.

DOI:10.1104/pp.46.6.831
PMID:16657552
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC396692/
Abstract

Plant cell walls contain a glycoprotein rich in hydroxyproline. To determine how Acer pseudoplatanus L. cells transport this glycoprotein to the wall, the pulse-chase technique was used to follow changes in specific radio-activity of hydroxyproline and proline in isolated, mitochondrial, Golgi, microsomal, soluble protein, and wall fractions. The turnover rates or changes in specific radioactivity of cytoplasmic hydroxyproline in these cell fractions indicated that the bulk of this hydroxyproline was transferred not by the Golgi apparatus but by a smooth membranous component.

摘要

植物细胞壁含有富含羟脯氨酸的糖蛋白。为了确定 Acer pseudoplatanus L. 细胞如何将这种糖蛋白运输到细胞壁，使用脉冲追踪技术来跟踪分离的线粒体、高尔基体、微粒体、可溶性蛋白质和细胞壁部分中羟脯氨酸和脯氨酸的放射性变化。这些细胞部分中细胞质羟脯氨酸的周转率或放射性变化表明，大部分羟脯氨酸不是通过高尔基体而是通过光滑的膜成分转移的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65fe/396692/0986555ff6d3/plntphys00204-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65fe/396692/cdc3d19eab31/plntphys00204-0071-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65fe/396692/0986555ff6d3/plntphys00204-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65fe/396692/cdc3d19eab31/plntphys00204-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65fe/396692/edf9b8cefba6/plntphys00204-0072-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65fe/396692/0986555ff6d3/plntphys00204-0075-a.jpg

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本文引用的文献

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2
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参与胡萝卜根切片中羟脯氨酸糖蛋白合成和运输的细胞器。
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