John Innes Institute, Colney Lane, Norwich, England.
Planta. 1972 Mar;107(1):43-51. doi: 10.1007/BF00398013.
In sycamore suspension cells both autoradiographical and chemical techniques have confirmed the idea that most of the protein that contains hydroxyproline is located within the cell wall. Relatively more hydroxyproline is incorporated into the walls of stationary phase cells than into those of cells actively engaged in cell division. It is probable that the protein enters the wall during and after the later stages of cell plate consolidation, and this may be facilitated by means of smooth membrane. Distinctive and heavy incorporation of radioactive proline is found associated with unusual wall thickenings or 'warts'.
在悬铃木悬浮细胞中,放射性自显影和化学技术都证实了这样一种观点,即大多数含有羟脯氨酸的蛋白质都位于细胞壁内。与积极参与细胞分裂的细胞相比,处于静止期的细胞的细胞壁中掺入了相对更多的羟脯氨酸。很可能蛋白质是在细胞板固着后期进入细胞壁的,并且这可能是通过光滑的膜来促进的。放射性脯氨酸的明显和大量掺入与不寻常的细胞壁增厚或“疣”有关。