Plantenfysiologisch Laboratorium, Universiteit van Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
Plant Physiol. 1981 Oct;68(4):910-3. doi: 10.1104/pp.68.4.910.
Sonication of a crude cell organelle fraction from hypocotyl tissue of dark-grown bean seedlings, and from suspension-cultured cells released a hydroxyproline-containing protein. The purification of this protein is described. It was found to be an arabinogalactan protein composed of 90% carbohydrate and 10% protein. The major sugars are galactose, arabinose, and uronic acids, and the major amino acids are hydroxyproline, serine, and alanine. Its molecular weight was estimated at 1.4 x 10(5) daltons and the isoelectric point at pH 2.3. The molecule is soluble in 5% trichloroacetic acid and can be precipitated with beta-galactosyl Yariv antigen. Pulse-chase experiments indicated that it was a secretory protein. The biosynthesis of arabinogalactan proteins is discussed.
超声处理暗培养的菜豆下胚轴组织和悬浮培养细胞释放的一种含有羟脯氨酸的蛋白粗细胞器官部分,本文描述了该蛋白的纯化方法。结果表明该蛋白是一种由 90%碳水化合物和 10%蛋白质组成的阿拉伯半乳聚糖蛋白。其主要糖为半乳糖、阿拉伯糖和糖醛酸,主要氨基酸为羟脯氨酸、丝氨酸和丙氨酸。其分子量估计为 1.4 x 10(5) 道尔顿,等电点为 pH 2.3。该分子可溶于 5%三氯乙酸,可被β-半乳糖基 Yariv 抗原沉淀。脉冲追踪实验表明它是一种分泌蛋白。讨论了阿拉伯半乳聚糖蛋白的生物合成。