Botany Department, University of California, Davis, California 95616.
Plant Physiol. 1971 Feb;47(2):189-91. doi: 10.1104/pp.47.2.189.
Nitrate reductase was not found to be present in or associated with partially purified, intact chloroplasts aqueously isolated from Wolffia arrhiza. Such chloroplasts are capable of using nitrite but not nitrate as an electron acceptor during light-stimulated electron transport in the absence of additional cytoplasmic components. When nitrite acts as an electron acceptor under these conditions, on the average 1.5 moles of oxygen are evolved per mole of nitrite reduced by the chloroplasts, indicating a probable reduction of nitrite to ammonia. Chloroplasts ruptured by osmotic shock fail to reduce nitrite in the absence of additional components.
硝酸还原酶未在从旱生狸藻中分离得到的、部分纯化的完整叶绿体或其相关部分中被发现。在没有细胞质成分的情况下，当叶绿体在光照下进行电子传递时，这些叶绿体能够利用亚硝酸盐而不是硝酸盐作为电子受体。当亚硝酸根在这些条件下作为电子受体时，叶绿体每还原 1 摩尔亚硝酸根平均释放 1.5 摩尔氧气，这表明亚硝酸根可能被还原为氨。渗透压休克破裂的叶绿体在没有其他成分的情况下不能还原亚硝酸盐。
