菠菜叶原生质体中苹果酸和磷酸二羟丙酮依赖的硝酸盐还原作用
Malate and Dihydroxyacetone Phosphate-dependent Nitrate Reduction in Spinach Leaf Protoplasts.
作者信息
Rathnam C K
机构信息
Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey 08903.
出版信息
Plant Physiol. 1978 Aug;62(2):220-3. doi: 10.1104/pp.62.2.220.
Abstract
Isolated spinach (Spinacia oleracea L. var. Bloomsdale) leaf protoplasts reduced nitrate at rates of 9 micromoles per milligram chlorophyll per hour in light with a 3- to 4-fold stimulation in the presence of HCO(3) (-). A similar stimulation of nitrate reduction in the absence of CO(2) fixation was obtained by the addition of malate, oxaloacetate (OAA), phospho-3-glyceric acid (PGA), or dihydroxyacetone phosphate (DHAP). Stimulation by malate and DHAP was light-independent, while the PGA and OAA effect was light-dependent. Nitrate reduction was found to be coupled to the cytoplasmic oxidation of DHAP or malate. The PGA/DHAP and OAA/malate shuttle across the chloroplast envelope has been demonstrated to support CO(2) fixation and/or nitrate reduction. The leaf protoplasts readily assimilated nitrate into amino-N in a stoichiometric relationship.
摘要
分离的菠菜（菠菜变种布洛姆斯代尔）叶原生质体在光照下以每毫克叶绿素每小时9微摩尔的速率还原硝酸盐，在存在HCO₃⁻的情况下有3至4倍的刺激作用。在不进行二氧化碳固定的情况下，通过添加苹果酸、草酰乙酸（OAA）、磷酸甘油酸（PGA）或磷酸二羟丙酮（DHAP）也能获得类似的硝酸盐还原刺激作用。苹果酸和DHAP的刺激作用不依赖光照，而PGA和OAA的作用则依赖光照。发现硝酸盐还原与DHAP或苹果酸的细胞质氧化相偶联。已证明PGA/DHAP和OAA/苹果酸穿梭穿过叶绿体包膜可支持二氧化碳固定和/或硝酸盐还原。叶原生质体很容易以化学计量关系将硝酸盐同化为氨基氮。
相似文献
1
Malate and Dihydroxyacetone Phosphate-dependent Nitrate Reduction in Spinach Leaf Protoplasts.菠菜叶原生质体中苹果酸和磷酸二羟丙酮依赖的硝酸盐还原作用
Plant Physiol. 1978 Aug;62(2):220-3. doi: 10.1104/pp.62.2.220.
2
Reversal of 3-(3,4-dichlorophenyl)-1,1-dimethylurea inhibition of carbon dioxide fixation in spinach chloroplasts and protoplasts by dicarboxylic acids.二羧酸逆转 3-(3,4-二氯苯基）-1,1-二甲基脲对菠菜叶绿体和原生质体中二氧化碳固定的抑制作用。
Plant Physiol. 1977 Jul;60(1):51-3. doi: 10.1104/pp.60.1.51.
3
Isolation of dihydroxyacetone phosphate reductase from dunaliella chloroplasts and comparison with isozymes from spinach leaves.从杜氏盐藻叶绿体中分离二羟丙酮磷酸还原酶并与菠菜叶片同工酶比较。
Plant Physiol. 1988 Nov;88(3):896-903. doi: 10.1104/pp.88.3.896.
4
Low CO(2) Prevents Nitrate Reduction in Leaves.低二氧化碳浓度会抑制叶片中的硝酸盐还原。
Plant Physiol. 1989 Nov;91(3):970-4. doi: 10.1104/pp.91.3.970.
5
Stimulation of ammonia and 2-oxoglutarate-dependent o(2) evolution in isolated chloroplasts by dicarboxylates and the role of the chloroplast in photorespiratory nitrogen recycling.二羧酸对分离叶绿体中氨和 2-氧戊二酸依赖性 O2 释放的刺激作用及叶绿体在光呼吸氮循环中的作用。
Plant Physiol. 1982 Mar;69(3):591-6. doi: 10.1104/pp.69.3.591.
6
On the Mechanism of Activation by Light of the NADP-dependent Malate Dehydrogenase in Spinach Chloroplasts.光激活菠菜叶绿体中依赖 NADP 的苹果酸脱氢酶的机制。
Plant Physiol. 1979 Nov;64(5):744-8. doi: 10.1104/pp.64.5.744.
7
Redox Transfer across the Inner Chloroplast Envelope Membrane.跨内叶绿体被膜的氧化还原传递。
Plant Physiol. 1991 Apr;95(4):1131-7. doi: 10.1104/pp.95.4.1131.
8
On the Function of Mitochondrial Metabolism during Photosynthesis in Spinach (Spinacia oleracea L.) Leaves (Partitioning between Respiration and Export of Redox Equivalents and Precursors for Nitrate Assimilation Products).菠菜（Spinacia oleracea L.）叶片光合作用过程中 mitochondrial metabolism 的功能（呼吸作用与硝酸盐同化产物氧化还原当量及前体输出之间的分配） 。
注：mitochondrial metabolism 可能有误，正常应该是“线粒体代谢”即“mitochondrial metabolism” ，这里按照你提供的原文翻译。
Plant Physiol. 1993 Dec;103(4):1147-1154. doi: 10.1104/pp.103.4.1147.
9
Hydrogen peroxide synthesis in isolated spinach chloroplast lamellae : an analysis of the mehler reaction in the presence of NADP reduction and ATP formation.离体菠菜叶绿体片层中过氧化氢的合成：在NADP还原和ATP形成存在下对梅勒反应的分析。
Plant Physiol. 1982 Nov;70(5):1249-54. doi: 10.1104/pp.70.5.1249.
10
Evidence for Endogenous Cyclic Photophosphorylation in Intact Chloroplasts: CO(2) Fixation with Dihydroxyacetone Phosphate.完整叶绿体中内源性循环光合磷酸化的证据：二羟丙酮磷酸的 CO2 固定。
Plant Physiol. 1983 Jun;72(2):321-5. doi: 10.1104/pp.72.2.321.
引用本文的文献
1
Photorespiration and nitrate assimilation: a major intersection between plant carbon and nitrogen.光呼吸与硝酸盐同化作用：植物碳代谢与氮代谢的一个主要交叉点
Photosynth Res. 2015 Feb;123(2):117-28. doi: 10.1007/s11120-014-0056-y. Epub 2014 Nov 4.
2
Competition between electron acceptors in photosynthesis: Regulation of the malate valve during CO2 fixation and nitrite reduction.光合作用中电子受体间的竞争：在 CO2 固定和亚硝酸盐还原过程中苹果酸阀的调节。
Photosynth Res. 1994 Oct;42(1):75-86. doi: 10.1007/BF00019060.
3
Ultrastructural demonstration of ferricyanide reductase (Diaphorase) activity in the envelopes of the plastids of etiolated barley (Hordeum vulgare L.) leaves.电镜下观察到暗培养大麦（Hordeum vulgare L.）叶片质体被膜中铁氰化物还原酶（黄递酶）的活性。
Planta. 1981 May;151(5):439-46. doi: 10.1007/BF00386537.
4
Photosynthesis of leaf cell protoplasts and permeability of the plasmalemma to some solutes.叶片细胞原生质体的光合作用和质膜对某些溶质的通透性。
Planta. 1983 Apr;157(5):462-70. doi: 10.1007/BF00397204.
5
Distribution of reducing power between photosynthetic carbon and nitrogen assimilation in Scenedesmus.在栅藻中，光合碳同化与氮同化之间还原力的分配。
Planta. 1985 May;164(2):246-53. doi: 10.1007/BF00396088.
6
Short-term effects of nitrate on sucrose synthesis in wheat leaves.硝态氮对小麦叶片蔗糖合成的短期影响。
Planta. 1991 Aug;185(1):53-7. doi: 10.1007/BF00194514.
7
Shift in carbon flow and stimulation of amino-acid turnover induced by nitrate and ammonium assimilation in Anacystis nidulans.硝酸根和铵同化诱导的鱼腥蓝细菌碳流和氨基酸周转的变化。
Planta. 1993 Mar;189(3):461-7. doi: 10.1007/BF00194446.
8
Nitrate-dependent o(2) evolution in intact leaves.完整叶片中依赖硝酸盐的氧气释放
Plant Physiol. 1991 Jul;96(3):898-901. doi: 10.1104/pp.96.3.898.
9
Low CO(2) Prevents Nitrate Reduction in Leaves.低二氧化碳浓度会抑制叶片中的硝酸盐还原。
Plant Physiol. 1989 Nov;91(3):970-4. doi: 10.1104/pp.91.3.970.
10
Photosynthetic Assimilation of NO(3) by Intact Cells of the Cyanobacterium Anacystis nidulans: Influence of NO(3) and NH(4) Assimilation on CO(2) Fixation.藻蓝蛋白完整细胞对硝酸盐（NO3-）的光合作用同化：硝酸盐和氨同化对二氧化碳固定的影响。
Plant Physiol. 1987 Jan;83(1):208-12. doi: 10.1104/pp.83.1.208.
本文引用的文献
1
Reversal of 3-(3,4-dichlorophenyl)-1,1-dimethylurea inhibition of carbon dioxide fixation in spinach chloroplasts and protoplasts by dicarboxylic acids.二羧酸逆转 3-(3,4-二氯苯基）-1,1-二甲基脲对菠菜叶绿体和原生质体中二氧化碳固定的抑制作用。
Plant Physiol. 1977 Jul;60(1):51-3. doi: 10.1104/pp.60.1.51.
2
Alpha-ketoglutarate supply for amino Acid synthesis in higher plant chloroplasts: intrachloroplastic localization of NADP-specific isocitrate dehydrogenase.高等植物叶绿体中用于氨基酸合成的α-酮戊二酸供应：NADP 特异性异柠檬酸脱氢酶的类囊体定位。
Plant Physiol. 1977 Apr;59(4):738-40. doi: 10.1104/pp.59.4.738.
3
Nitrite Reduction in Reconstituted and Whole Spinach Chloroplasts during Carbon Dioxide Reduction.在二氧化碳还原过程中，重组和完整菠菜叶绿体中的亚硝酸盐还原。
Plant Physiol. 1977 Feb;59(2):184-8. doi: 10.1104/pp.59.2.184.
4
Distribution of Nitrate-assimilating Enzymes between Mesophyll Protoplasts and Bundle Sheath Cells in Leaves of Three Groups of C(4) Plants.三组C4植物叶片中叶肉原生质体与维管束鞘细胞间硝酸盐同化酶的分布
Plant Physiol. 1976 Jun;57(6):881-5. doi: 10.1104/pp.57.6.881.
5
Site of action of inhibitors of carbon dioxide assimilation by whole lettuce chloroplasts.完整生菜叶绿体二氧化碳同化抑制剂的作用位点。
Plant Physiol. 1975 Oct;56(4):481-5. doi: 10.1104/pp.56.4.481.
6
Dependence of nitrite reduction on electron transport chloroplasts.亚硝酸盐还原对叶绿体电子传递的依赖性。
Plant Physiol. 1974 Oct;54(4):480-3. doi: 10.1104/pp.54.4.480.
7
Nitrite assimilation and amino nitrogen synthesis in isolated spinach chloroplasts.离体菠菜叶绿体中亚硝酸盐同化和氨基酸氮合成。
Plant Physiol. 1974 Mar;53(3):411-5. doi: 10.1104/pp.53.3.411.
8
Purification of enzymatically isolated mesophyll protoplasts from c(3), c(4), and crassulacean Acid metabolism plants using an aqueous dextran-polyethylene glycol two-phase system.使用葡聚糖 - 聚乙二醇水相两相系统从C(3)、C(4)和景天酸代谢植物中酶法分离叶肉原生质体的纯化
Plant Physiol. 1973 Nov;52(5):484-90. doi: 10.1104/pp.52.5.484.
9
Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves.绿叶中用于硝酸盐还原的还原型烟酰胺腺嘌呤二核苷酸的生成。
Plant Physiol. 1971 Nov;48(5):580-90. doi: 10.1104/pp.48.5.580.
10
Nitrate and Nitrite Reduction by Wolffia arrhiza.Wolffia arrhiza 对硝酸盐和亚硝酸盐的还原作用。
Plant Physiol. 1971 Feb;47(2):189-91. doi: 10.1104/pp.47.2.189.
Zotero 插件