Jones R L
Department of Botany, University of California, Berkeley, California 94720.
Plant Physiol. 1971 Mar;47(3):412-6. doi: 10.1104/pp.47.3.412.
A beta-1, 3-glucanase of barley (Hordeum vulgare) aleurone cells accumulates when half-seeds are imbibed on water, and accumulation continues when the aleurone layers are incubated in buffer solution. The release of the enzyme is a gibberellic acid-dependent process, however. Although gibberellic acid stimulates glucanase release, it does not markedly affect the total amount of glucanase obtained from these cells when compared with water controls. beta-1, 3-Glucanase release from aleurone cells is a function of gibberellic acid concentration and commences after a 4-hour lag period. Processes occurring during this lag period are also dependent upon gibberellic acid concentration. Removal of gibberellic acid from the incubation medium at the end of the lag period, however, does not affect subsequent release of glucanase. The release of glucanase from aleurone cells is an active process with a Q(10) greater than 3. Inhibitors of respiration and protein and RNA synthesis effectively inhibit the formation and release of glucanase. It is concluded that gibberellic acid functions primarily to enhance glucanase release rather than its formation.
大麦（Hordeum vulgare）糊粉层细胞的β-1,3-葡聚糖酶在半粒种子吸水时积累，并且当糊粉层在缓冲溶液中培养时积累仍会继续。然而，该酶的释放是一个依赖赤霉素的过程。尽管赤霉素刺激葡聚糖酶的释放，但与用水作为对照相比，它对从这些细胞中获得的葡聚糖酶总量没有显著影响。糊粉层细胞中β-1,3-葡聚糖酶的释放是赤霉素浓度的函数，并且在4小时的延迟期后开始。在此延迟期内发生的过程也取决于赤霉素浓度。然而，在延迟期结束时从培养介质中去除赤霉素，并不影响随后葡聚糖酶的释放。糊粉层细胞中葡聚糖酶的释放是一个Q(10)大于3的活跃过程。呼吸、蛋白质和RNA合成的抑制剂能有效抑制葡聚糖酶的形成和释放。得出的结论是，赤霉素的主要作用是增强葡聚糖酶的释放而不是其形成。
