Department of Botany, University of California, Berkeley, California 94720.
Plant Physiol. 1973 Oct;52(4):303-8. doi: 10.1104/pp.52.4.303.
The release of potassium, magnesium, and phosphate ions from aleurone cells of barley (Hordeum vulgare L. cv. Himalaya) is a gibberellic acid-dependent process. The release of these ions is preceded by a lag period of 6 to 8 hours after gibberellic acid addition. The effect of gibberellic acid on the release of ions is not mediated through an effect on ion solubilization. Thus, gibberellic acid does not apreciably affect the sum of extracted and released ions relative to controls. Rather, the effect of the hormone is on the release process itself. Inhibitors of oxidative phosphorylation when added with gibberellic acid or at times up to 6 hours after gibberellic acid inhibition release. When these inhibitors are added after ion release has begun, however, rapid efflux of ions occurs. These results suggest a strong correlation between energy levels and ion transport capacity. Inhibitors of RNA and protein synthesis also inhibit gibberellic acid-stimulated ion release. Evidence suggests that RNA and protein synthesis are required to establish and maintain ion release capacity of aleurone cells.
从大麦（Hordeum vulgare L. cv. Himalaya）糊粉细胞中释放钾、镁和磷酸盐离子是赤霉素依赖的过程。在添加赤霉素 6 到 8 小时后，才会出现离子释放的滞后期。赤霉素对离子释放的影响不是通过对离子溶解的影响来介导的。因此，赤霉素不会相对于对照物显著影响提取和释放离子的总和。相反，激素的作用是在释放过程本身。当与赤霉素一起添加或在赤霉素抑制后长达 6 小时添加氧化磷酸化抑制剂时，会释放离子。然而，当这些抑制剂在离子释放开始后添加时，离子会迅速流出。这些结果表明能量水平和离子运输能力之间存在很强的相关性。RNA 和蛋白质合成抑制剂也会抑制赤霉素刺激的离子释放。有证据表明，RNA 和蛋白质合成是建立和维持糊粉细胞离子释放能力所必需的。
