Honeycutt R C, Krogmann D W
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1972 Mar;49(3):376-80. doi: 10.1104/pp.49.3.376.
Phenylmercuric acetate is a selective inhibitor of the photosynthetic activities of isolated spinach (Spinacia oleracea) chloroplasts. At 5 mum concentration of phenylmercuric acetate, photophosphorylation is inhibited. At 33 mum phenylmercuric acetate, ferredoxin is inactivated. Ferredoxin-NADP oxidoreductase is 50% inhibited at 100 mum phenylmercuric acetate. Photosystem II reactions are 50% inhibited at 150 mum phenylmercuric acetate and very much higher cooncentrations-500 mum-are needed to approach complete inhibition. Phenylmercuric acetate inhibition of photosystem II appears to be selective, blocking a site between the 3-(3,4-dichlorophenyl)-1,1-dimethyl urea sensitive site and the site inactivated by high concentrations of tris buffer.
醋酸苯汞是分离的菠菜叶绿体光合活性的选择性抑制剂。在醋酸苯汞浓度为5微摩尔时，光合磷酸化受到抑制。在醋酸苯汞浓度为33微摩尔时，铁氧化还原蛋白失活。在醋酸苯汞浓度为100微摩尔时，铁氧化还原蛋白-NADP氧化还原酶受到50%的抑制。在醋酸苯汞浓度为150微摩尔时，光系统II反应受到50%的抑制，而要达到完全抑制则需要更高得多的浓度——500微摩尔。醋酸苯汞对光系统II的抑制似乎具有选择性，阻断了在3-(3,4-二氯苯基)-1,1-二甲基脲敏感位点和高浓度三羟甲基氨基甲烷缓冲液使其失活的位点之间的一个位点。
