Schwelitz F D, Dilley R A, Crane F L
Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387.
Plant Physiol. 1972 Jul;50(1):166-70. doi: 10.1104/pp.50.1.166.
The techniques of thin sectioning and freeze etching were employed in comparing the chloroplast structure of the wild type and photosynthetic mutant P(4) of Euglena gracilis, Z strain. The mutant chloroplasts were characterized by a lack of thylakoid pairing even under high salt conditions. In addition the mutant thylakoids were more varied in size and fewer in number than those of the wild type. No differences between the mutant and wild type were observed in the size and distribution of the particles within the chloroplast membranes seen by the freeze-etching technique.These structural abnormalities do not appear to be correlated per se with the absence of plastoquinone A in the mutant but may be related to a different lipid composition observed in the mutant.
运用超薄切片和冷冻蚀刻技术，比较了纤细裸藻Z菌株野生型和光合突变体P(4)的叶绿体结构。突变体叶绿体的特征是，即使在高盐条件下，类囊体也不会配对。此外，突变体类囊体的大小变化更大，数量比野生型更少。通过冷冻蚀刻技术观察发现，突变体和野生型叶绿体膜内颗粒的大小和分布没有差异。这些结构异常本身似乎与突变体中质体醌A的缺失无关，但可能与突变体中观察到的不同脂质组成有关。
