Stevens S E, Van Baalen C
University of Texas Marine Science Institute, Port Aransas, Texas 78373.
Plant Physiol. 1973 Feb;51(2):350-6. doi: 10.1104/pp.51.2.350.
Characteristics of nitrate reduction in terms of nitrite production in an N-methyl-N'-nitro-N-nitrosoguanidine-induced mutant of the blue-green alga Agmenellum quadruplicatum are described. Following induction of nitrate reduction a linear rate of nitrite production proportional to cell concentration was observed. Rate of nitrite production and growth rate showed similar responses to pH, temperature, and light intensity. If required, only trace amounts of carbon dioxide were necessary for nitrite production. Atmospheres of oxygen or nitrogen inhibited production of nitrite. In addition, a low but constant rate of nitrite production was observed in the dark. Nitrite production by mutant AQ-6 was studied in terms of photosynthesis. As nitrite production proceeded, rate of photosynthesis declined. Ultraviolet irradiation and 3-(3,4-dichlorophenyl)-1, 1-dimethylurea poisoning did not prevent nitrite production. The action spectrum of nitrite production was chlorophyll a-like.
描述了在N-甲基-N'-硝基-N-亚硝基胍诱导的蓝藻四倍体Agmenellum突变体中，硝酸盐还原过程中亚硝酸盐产生的特征。诱导硝酸盐还原后，观察到亚硝酸盐产生的线性速率与细胞浓度成正比。亚硝酸盐产生速率和生长速率对pH、温度和光照强度表现出相似的响应。如果需要，仅需微量二氧化碳即可进行亚硝酸盐产生。氧气或氮气气氛会抑制亚硝酸盐的产生。此外，在黑暗中观察到亚硝酸盐产生速率较低但恒定。从光合作用的角度研究了突变体AQ-6的亚硝酸盐产生。随着亚硝酸盐产生的进行，光合作用速率下降。紫外线照射和3-(3,4-二氯苯基)-1,1-二甲基脲中毒并不能阻止亚硝酸盐的产生。亚硝酸盐产生的作用光谱类似叶绿素a。
